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      FITC Mouse IgM, κ Isotype Control
      FITC Mouse IgM, κ Isotype Control
      Flow cytometric analysis of FITC Mouse IgM, κ Isotype Control expression on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta). Whole blood was stained with either FITC Mouse IgM, κ Isotype Control (Cat. No. 551448; solid line histogram) or left unstained (dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
      FITC Mouse IgM, κ Isotype Control
      Flow cytometric analysis of FITC Mouse IgM, κ Isotype Control expression on peripheral blood lymphocytes of Rhesus macaque (macaca mulatta). Whole blood was stained with either FITC Mouse IgM, κ Isotype Control (Cat. No. 551448; solid line histogram) or left unstained (dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
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      BD Pharmingen™
      Anti-TNP
      Rhesus, Cynomolgus, Baboon (QC Testing)
      Mouse BALB/c IgM, κ
      TNP-Keyhole Limpet Hemocyanin
      Flow cytometry, Isotype control (Routinely Tested)
      20 µl
      AB_394198
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      551448 Rev. 4
      Antibody Details
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      G155-228

      The G155-228 antibody is specific for the hapten trinitrophenol (TNP), a hapten not expressed on human, non-human primate, mouse, or rat primary cells or cell lines. In the absence of specific binding, this immunoglobulin may bind non-specifically to Fc receptors.

      This reagent has been used as isotype control in immunophenotypic studies with baboon, rhesus, or cynomolgus peripheral blood cells.

      551448 Rev. 4
      Format Details
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      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      551448 Rev.4
      Citations & References
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      View product citations for antibody "551448" on CiteAb
      No Citations Found

      No Citations are available for this Product

      551448 Rev. 4

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.