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Purified Mouse Anti-Human CD10
Product Details
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BD™
Human
Mouse BALB/c IgG2a, κ
Blasts from a donor with non-T ALL
Flow cytometry
6 μg/mL
20 μL
4311
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347500 Rev. 1
Antibody Details
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W8E7

The CD10 antibody, clone W8E7, is derived from the hybridization of P3-X63-Ag8.653 mouse myeloma cells with spleen cells of BALB/c mice immunized with blasts from a patient with non-T ALL.

The CD10 antibody (Anti-CALLA) recognizes a human common acute lymphoblastic leukemia antigen (CALLA), with a molecular weight of 100 kilodaltons (kDa). The CD10 antigen is identical to human membrane–associated neutral endopeptidase (NEP; EC 3.3.24.11), also known as enkephalinase.

347500 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
347500 Rev.1
Citations & References
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View product citations for antibody "347500" on CiteAb

Development References (11)

  1. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Dworzak MN, Fritsch G, Fleischer C, et al. Multiparameter phenotype mapping of normal and post-chemotherapy B lymphopoiesis in pediatric bone marrow.. Leukemia. 1997; 11(8):1266-73. (Biology). View Reference
  4. Dworzak MN, Fritsch G, Fröschl G, Printz D, Gadner H. Four-color flow cytometric investigation of terminal deoxynucleotidyl transferase-positive lymphoid precursors in pediatric bone marrow: CD79a expression precedes CD19 in early B-cell ontogeny.. Blood. 1998; 92(9):3203-9. (Biology). View Reference
  5. Greaves MF. Knapp W, ed. Leukemia Markers. New York, NY: Academic Press; 1981:19.
  6. Hann IM, Richards SM, Eden OB, Hill FG. Analysis of the immunophenotype of children treated on the Medical Research Council United Kingdom Acute Lymphoblastic Leukaemia Trial XI (MRC UKALLXI). Medical Research Council Childhood Leukaemia Working Party.. Leukemia. 1998; 12(8):1249-55. (Biology). View Reference
  7. LeBien TW, McCormack RT. The common acute lymphoblastic leukemia antigen (CD10)--emancipation from a functional enigma.. Blood. 1989; 73(3):625-35. (Biology). View Reference
  8. Letarte M, Vera S, Tran R, et al. Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase. J Exp Med. 1988; 168(4):1247-1253. (Biology). View Reference
  9. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
  10. Nadler LM, Korsmeyer SJ, Anderson KC, et al. B cell origin of non-T cell acute lymphoblastic leukemia. A model for discrete stages of neoplastic and normal pre-B cell differentiation.. J Clin Invest. 1984; 74(2):332-40. (Biology). View Reference
  11. Zola H. CD10 Workshop Panel report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:505-507.
View All (11) View Less
347500 Rev. 1

 

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