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PE Mouse Anti-Human CD38
Product Details
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BD Quantibrite™
T10; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; OKT10
Human
Mouse IgG1, κ
Human BJAB B cell line
Flow cytometry
12.5 μg/mL
20 μL
III B918
952
AB_400453
Phosphate buffered saline with BSA and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The QuantiBRITE CD38 PE (≥95% 1:1 PE:mAb ratio) reagent is supplied as 12.5 μg in 1 mL (12.5 μg/mL) of PBS containing bovine serum albumin and 0.1% sodium azide. Vials should be stored at 2–8°C. QuantiBRITE reagents should not be frozen and should be protected from prolonged exposure to light. The reagent is stable for the period shown on the bottle label when stored as directed.

342371 Rev. 1
Antibody Details
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HB7

The CD38 antibody, clone HB7, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the BJAB cell line.

The CD38 antibody recognizes an integral membrane glycoprotein of 45 kilodaltons (kDa), with a protein core of 35 kDa. The CD38 antigen is also known as T10, ADP-ribosyl cyclase, and cyclic ADP ribose hydrolase 1.

342371 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
342371 Rev.1
Citations & References
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View product citations for antibody "342371" on CiteAb

Development References (25)

  1. Babusikova O, Ondrackova V, Prachar J, Kusenda J, Hraska V. Flow cytometric analysis of some activation/proliferation markers on human thymocytes and their correlation with cell proliferation. Neoplasma. 1999; 46(6):349-355. (Biology).
  2. Badley AD, Parato K, Cameron DW, et al. Dynamic correlation of apoptosis and immune activation during treatment of HIV infection. Cell Death Differ. 1999; 6(5):420-432. (Biology).
  3. Damle RN, Wasil T, Fais F, et al. Ig V gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia. Blood. 1999; 94(6):1840-1847. (Biology).
  4. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, et al. Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989:86.
  5. Giorgi J, Hultin L. Lymphocyte subset alterations and immunophenotyping by flow cytometry in HIV disease. Clin Immunol Newslett. 1990; 10(4):55-61. (Biology).
  6. Giorgi JV, Detels R. T-cell subset alterations in HIV-infected homosexual men: NIAID multicenter AIDS cohort study. Clin Immunol Immunopathol. 1989; 52:10-18. (Biology).
  7. Hultin LE, Matud JL, Giorgi JV. Quantitation of CD38 activation antigen expression on CD8+ T cells in HIV-1 infection using CD4 expression on CD4+ T lymphocytes as a biological calibrator. Cytometry. 1998; 33(2):123-132. (Biology).
  8. Inoue K, Ogawa H, Sonoda Y, et al. Aberrant overexpression of the Wilms tumor gene (WT1) in human leukemia. Blood. 1997; 89(4):1405-1412. (Biology).
  9. Iyer SB, Hultin LE, Zawadzki JA, Davis KA, Giorgi JV Quantitation of CD38 expression using QuantiBRITE beads. Cytometry. 1998; 33(2):206-212. (Biology).
  10. Kawagoe H, Humphries RK, Blair A, Sutherland HJ, Hogge DE. Expression of HOX genes, HOX cofactors, and MLL in phenotypically and functionally defined subpopulations of leukemic and normal human hematopoietic cells. Leukemia. 1999; 13(5):687-698. (Biology).
  11. Landay A, Ohlsson-Wilhelm B, Giorgi JV. Application of flow cytometry to the study of HIV infection. AIDS. 1990; 4(6):479-497. (Biology). View Reference
  12. Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). View Reference
  13. Lenki R, Bratt G, Holmberg V, Muirhead K, Sandstrom E. Indicators of T-cell activation: Correlation between quantitative CD38 expression and soluble CD8 levels in asymptomatic HIV+ individuals and healthy controls. Cytometry. 1998; 33:115-122. (Biology).
  14. Otawa M, Kawanishi Y, Iwase O, Shoji N, Miyazawa K, Ohyashiki K. Comparative multi-color flow cytometric analysis of cell surface antigens in bone marrow hematopoietic progenitors between refractory anemia and aplastic anemia. Leuk Res. 2000; 24(4):359-366. (Biology).
  15. Perfetto SP, Malone JD, Hawkes C, et al. CD38 expression on cryopreserved CD8+ T cells predicts HIV disease progression. Cytometry. 1998; 33(2):133-137. (Biology).
  16. Pezzutto A, Behm F, Callard RE. Flow cytometry analysis of the B-cell blind panel: joint report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:165-174.
  17. Rawstron AC, Owen RG, Davies FE, et al. Circulating plasma cells in multiple myeloma: characterization and correlation with disease stage. Br J Haematol. 1997; 97:46-55. (Biology).
  18. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980; 77(3):1588-1592. (Biology). View Reference
  19. Schmitz JL, Czerniewski MA, Edinger M, et al. Multisite comparison of methods for the quantitation of the surface expression of CD38 on CD8(+) T lymphocytes. Cytometry. 2000; 42(3):174-179. (Biology).
  20. Shinjo K, Takeshita A, Higuchi M, Ohnishi K, Ohno R. Erythropoietin receptor expression on human bone marrow erythroid precursor cells by a newly-devised quantitative flow-cytometric assay. Br J Haematol. 1997; 96(3):551-558. (Biology).
  21. Tedder TF, Clement LT, Cooper MD. Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens. 1984; 24(3):140-149. (Biology). View Reference
  22. Tedder TF, Crain MJ, Kubagawa H, Clement LT, Cooper MD. Evaluation of lymphocyte differentiation in primary and secondary immunodeficiency diseases. J Immunol. 1985; 135(3):1785-1791. (Biology). View Reference
  23. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). View Reference
  24. Terstappen LW, Huang S, Picker LJ. Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow. Blood. 1992; 79(3):666-677. (Biology). View Reference
  25. Terstappen LW, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood. 1991; 77(6):1218-1227. (Biology). View Reference
View All (25) View Less
342371 Rev. 1

 

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