PE Mouse Anti-Human CD103

BD™ PE Mouse Anti-Human CD103

Name: PE Mouse Anti-Human CD103
Brand: BD™
SKU: 340998

Clone Ber-ACT8

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: ITGAE; Integrin alpha-E; ITAE; HML-1 antigen; Mucosal lymphocyte 1; HUMINAE

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human MAPS16 T Cell Line

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V A067, BP047, S256

Entrez Gene ID: 3682

Storage Buffer: Phosphate buffered saline with gelatin and sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340998 Rev. 1

Antibody Details

Ber-ACT8

The CD103 antibody, clone Ber-ACT8, is derived using standard cell hybridization procedures using spleen cells from BALB/c mice immunized with the HTLV-1–positive T-cell line, MAPS16.

The CD103 antibody recognizes the αE subunit of integrin αEβ

, an integrin also known as the human mucosal lymphocyte (HML) antigen 1, but not α, another member of the βintegrin subfamily. Integrin αEβis a trimeric protein complex of three components, 105 kilodaltons (kDa) (β), 150 kDa, and 25 kDa. The CD103 antibody recognizes an epitope localized on the 150-kDa chain.

340998 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340998 Rev.1

Citations & References

View product citations for antibody "340998" on CiteAb

Development References (14)

Brew R, West DC, Burthem J, Christmas SE. Expression of the human mucosal lymphocyte antigen, HML-1, by T cells activated with mitogen or specific antigen in vitro. Scan J Immunol. 1995; 41:553–562. (Biology).
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Cerf-Benussan N, Jarry A, Brousse N, Lisowska-Grospierre B, Guy-Grand D, Griscelli C. A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur J Immunol. 1987; 17:1279–1285. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Falini B, Pileri SA, Flenghi L, et al. Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients. Br J Haematol. 1990; 76:460–468. (Biology).
Hadley GA, Rostapshova EA, Gomolka DM, et al. Regulation of the epithelial cell-specific integrin, CD103, by human CD8+ cytolytic T lymphocytes. Transplantation. 1999; 67:1418–1425. (Biology).
Hassan IB, Hagberg H, Sundström C. Immunophenotype of hairy-cell leukemia. Eur J Haematol. 1990; 45:172-176. (Biology).
Kruschwitz M, Fritzsche G, Schwarting R, et al. Ber-ACT8: new monoclonal antibody to the mucosa lymphocyte antigen. J Clin Pathol. 1991; 44(8):636-645. (Biology). View Reference
Lohmeyer J, Friedrish J, Grimminger F, et al. Expression of mucosa-related integrin αEβ7 on alveolar T cells in interstitial lung diseases. Clin Exp Immunol. 1999; 116:340-346. (Biology).
Micklem KJ, Dong Y, Willis A, et al. HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the β7 subunit. Am J Pathol. 1996; 3:30-36. (Biology).
Moldenhauer G, Mielke B, Dörken B, Schwartz-Albiez R, Möller P. Identity of HML-1 antigen on intestinal intraepithelial T cells and of B-ly7 antigen on hairy cell leukaemia. Scand J Immunol. 1990; 32:77–82. (Biology).
Möller P, Mielke B, Moldenhauer G. Monoclonal antibody HML-1, a marker for intraepithelial T cells and lymphomas derived thereof, also recognizes hairy cell leukemia and some B-cell lymphomas. Am J Pathol. 1990; 136:509–512. (Biology).
Parker CM, Cepek KL, Russell GJ, et al. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(5):1924-1928. (Biology). View Reference
Rihs S, Walker C, Virchow JC Jr, et al. Differential expression of α E β 7 integrins on bronchoalveolar lavage T lymphocyte subsets: regulation by α 4 β 1-integrin crosslinking and TGF-β. Am J Respir Cell Mol Biol. 1996; 15:600–610. (Biology).

View All (14)

340998 Rev. 1

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