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APC-R700 Mouse Anti-Human CD200
Product Details
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BD Horizon™
OX-2; MOX1; MOX2 ; My033
Human
Mouse IgG1, κ
Human CD200 Recombinant Protein
Flow cytometry
50 μg/mL
5 μL
VII 70655; IX 40
4345
Phosphate buffered saline with BSA and ProClin®.
RUO (GMP)


Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

657868 Rev. 1
Antibody Details
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MRC OX-104

The CD200 antibody, clone MRC OX-104, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells isolated from a mouse immunized with a recombinant fusion protein containing the two IgSF domains comprising the human CD200 extracellular region.

The CD200 antibody specifically binds to a 40–45 kilodalton (kDa) type 1 transmembrane glycoprotein containing two immunoglobulin superfamily domains (IgSF), and a short cytoplasmic tail. The CD200 antigen is also known as OX-2, MOX1, and MOX2.

657868 Rev. 1
Format Details
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APC-R700
The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC-R700
Red 627-640 nm
651 nm
706 nm
657868 Rev.1
Citations & References
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View product citations for antibody "657868" on CiteAb

Development References (11)

  1. Barclay AN, Wright GJ, Brown MH. CD200 (OX2) Summary and Workshop report: The lymphoid/ neuronal OX2 glycoprotein (CD200) interacts with a novel receptor on macrophages. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:471-473.
  2. Borriello F, Lederer J, Scott S, Sharpe AH. MRC OX-2 defines a novel T cell co-stimulatory pathway. J Immunol. 1997; 158:4548-4554. (Biology).
  3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  4. Clark MJ, Gagnon J, Williams AF, Barclay AN. MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain. EMBO J. 1985; 4:113-118. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Hoek RM, Ruuls SR, Murphy CA, et al. Down-regulation of the macrophage lineage through interaction with OX2 (CD200).. Science. 2000; 290(5497):1768-71. (Biology). View Reference
  7. Wong KK, Brenneman F, Chesney A, Spaner DE, Gorczynski RM. Soluble CD200 is critical to engraft chronic lymphocytic leukemia cells in immunocompromised mice. Cancer Res. 2012; 72:4931-4943. (Biology).
  8. Wright GJ, Jones M, Puklavec MJ, Brown MH, Barclay AN. The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans. Immunology. 2001; 102(2):173-179. (Biology). View Reference
  9. Wright GJ, Puklavec MJ, Willis AC, et al. Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. Immunity. 2000; 13(2):233-242. (Biology). View Reference
  10. Zhang S, Cherwinski H, Sedgwick JD, Phillips JH. Molecular mechanisms of CD200 inhibition of mast cell activation. J Immunol. 2004; 173:6786-6793. (Biology).
  11. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference
View All (11) View Less
657868 Rev. 1

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