Anti-Human COX-1 FITC/COX-2 PE

BD™ Anti-Human COX-1 FITC/COX-2 PE

Name: Anti-Human COX-1 FITC/COX-2 PE
Brand: BD™
SKU: 334090

(RUO (GMP))

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Scientific Resources

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Reactivity: Human

Application: Flow cytometry

Regulatory Status: RUO (GMP)

Storage Buffer: Phosphate buffered saline with BSA, beta-lactoglobulin, and 0.1% sodium azide.

Description

Anti–COX-1, clone AS70, is an antibody raised against human recombinant COX-1 protein. The antibody does not cross-react with human recombinant COX-2 by ELISA.

Anti–COX-2, clone AS67, is an antibody raised against human recombinant COX-2 protein. The antibody does not cross-react with human recombinant COX-1 by ELISA.

Anti–COX-1 recognizes a homodimeric, heme-containing, glycosylated, constitutively expressed enzyme that is located on the lumenal surface of the endoplasmic reticulum and on the inner and outer membranes of the nuclear envelope. Anti–COX-2 recognizes the inducible form of this enzyme.

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

334090 Rev. 1

Components

Description	Clone	Isotype	EntrezGene ID
Anti-COX-1 FITC	N/A	N/A	N/A
PE Mouse Anti-Human COX-2	AS67	IgG1, κ	5743

334090 Rev. 1

Citations & References

Development References (9)

Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Fletcher BS, Kujubu DA, Perrin DM, Herschman HR. Structure of the mitogen-inducible TIS10 gene and demonstration that the TIS10-encoded protein is a functional prostaglandin G/H synthase. J Biol Chem. 1992; 267:4338-4344. (Biology).
Herschman HR. Prostaglandin synthase 2. Biochim Biophys Acta. 1996; 1299:125-140. (Biology).
Kang RY, Freire-Moar J, Sigal E, Chu C-Q. Expression of cyclooxygenase-2 in human and an animal model of rheumatoid arthritis. Br J Rheumatology. 1996; 35:711-718. (Biology).
Maloney CG; Kutchera WA; Albertine KH; McIntyre TM; Prescott SM; Zimmerman GA. Inflammatory agonists induce cyclooxygenase type 2 expression by human neutrophils. J Immunol. 1998; 160(3):1402-1410. (Biology). View Reference
Ruitenberg JJ; Waters CA. A rapid flow cytometric method for the detection of intracellular cyclooxygenases in human whole blood monocytes and a COX-2 inducible human cell line. J Immunol Methods. 2003; 274(1-2):93-104. (Biology). View Reference
Smith WL, Garavito RM, DeWitt DL. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J Biol Chem. 1996; 271:33157-33160. (Biology).
Spencer AG, Woods JW, Arakawa T, Singer II, Smith WL. Subcellular localization of prostaglandin endoperoxide H synthases-1 and -2 by immunoelectron microscopy. J Biol Chem. 1998; 273:9886-9893. (Biology).

View All (9)

334090 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.