RB705 Mouse Anti-Human CD159C (NKG2C)

BD OptiBuild™ RB705 Mouse Anti-Human CD159C (NKG2C)

Name: RB705 Mouse Anti-Human CD159C (NKG2C)
Brand: RB705 Mouse Anti-Human CD159C (NKG2C)
SKU: 756941

Clone 134591 (RUO)

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Catalog No:756941

Unit:EA (1 Each)

Size:

50 µg

Quantity:

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Product Details

Brand: BD OptiBuild™

Alternative Name: CD159c; KLRC2; killer cell lectin-like receptor subfamily C, member 2; NK cell receptor C; NKG2-C; NKG2C

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG1, κ

Immunogen: Human NKG2C/CD159c and CD94 Transfected Cell Line

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Workshop Number: VIII 80694

Entrez Gene ID: 3822

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Data Sheets

Technical Data Sheet (TDS)

756941 Rev. 1

Antibody Details

134591

The 134591 monoclonal antibody specifically recognizes CD159 antigen-like family member C (CD159c) which is also known as NKG2-C type II integral membrane protein or NKG2-C-activating NK receptor (NKG2C), or NK cell receptor C. CD159c (NKG2C) is a type II transmembrane glycoprotein that is encoded by KLRC2 (killer cell lectin like receptor C2) which belongs to the killer cell lectin-like receptor (KLR) family. CD159c (NKG2C) contains a C-type lectin domain in its extracellular region and is expressed by NK cells and some T cells. CD159c (NKG2C) can form a disulfide-bonded heterodimer with CD94 that non-covalently associates with DAP12 homodimers to form a functional signaling receptor complex. CD159c (NKG2C) binds to MHC class I HLA-E molecules on target cells to regulate NK cell activation.

756941 Rev. 1

Format Details

RB705

The BD Horizon RealBlue™ 705 (RB705) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 707-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB705 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB705 can be used as an alternative to PerCP-Cy5.5 or BB700 and we recommend using an optical filter centered near 710-nm (e.g., a 695/40 or 710/50-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PerCP-Cy5.5. RB705 is on average brighter than PerCP-Cy5.5 and BB700, and has minimal spillover into Yellow-Green detectors.

Format: RB705

Excitation Max: 498 nm

Emission Max: 707 nm

756941 Rev.1

Citations & References

No Citations Are Available for this Product

756941 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.