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Multicolor flow cytometric analysis of mouse CD371 (Clec12A) expression on spleen dendritic cell subsets. BALB/c splenocytes were simultaneously stained with V450 Rat Anti-Mouse CD45R (Cat. No. 560472), PerCP-Cy™5.5 Hamster Anti-Mouse CD11c (Cat. No. 560584), Alexa Fluor® 488 Hamster Anti-Mouse CD3e (Cat. No. 557666), APC-H7 Rat Anti-Mouse CD19 (Cat. No. 560245), APC Rat Anti-Mouse CD49b (Cat. No. 560628) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930, dashed line histogram) or PE Rat Anti-Mouse CD371 (Clec12A) (Cat. No. 562773, solid line histogram). Overlapping histograms show the levels of CD371 or Ig Isotype control staining in CD11c-high CD45R-negative (Left Panel) and CD11c-intermediate CD45R-positive (Right Panel) populations. Fluorescence histograms were derived from gated CD3- CD19- CD49b- events with the forward and side light-scattering characteristics of viable splenocytes cells. Flow cytometry was performed on a BD™ LSR II.
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BD Pharmingen™ PE Rat anti-Mouse CD371 (Clec12A)
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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 5D3/CD371 (aka, 1/06-5D3) monoclonal antibody specifically binds to mouse CD371 which is encoded by Clec12A (C-type lectin domain family 12 member A). CD371 is also known as Myeloid inhibitory C-type lectin-like receptor (MICL), C-type lectin-like molecule 1 (CLL-1) or Dendritic cell-associated lectin 2 (DCAL-2). CD371 is a ~30 kDa type II transmembrane glycoprotein that possesses one extracellular C-type lectin domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). CD371 is expressed on plasmacytoid dendritic cells, subsets of myeloid dendritic cells, monocytes, macrophages and B cells. It reportedly serves as a negative regulator of granulocyte and monocyte function.

Development References (3)
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Marshall AS, Willment JA, Lin HH, Williams DL, Gordon S, Brown GD. Identification and characterization of a novel human myeloid inhibitory C-type lectin-like receptor (MICL) that is predominantly expressed on granulocytes and monocytes.. J Biol Chem. 2004; 279(15):14792-802. (Biology). View Reference
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Marshall AS, Willment JA, Pyz E, et al. Human MICL (CLEC12A) is differentially glycosylated and is down-regulated following cellular activation.. Eur J Immunol. 2006; 36(8):2159-69. (Biology). View Reference
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van Rhenen A, van Dongen GA, Kelder A, et al. The novel AML stem cell associated antigen CLL-1 aids in discrimination between normal and leukemic stem cells.. Blood. 2007; 110(7):2659-66. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.