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BV421 Mouse Anti-Human TCR Vβ23

BD Horizon™ BV421 Mouse Anti-Human TCR Vβ23

Clone AHUT7 (also known as AF23; AF-23; αHUT7; HUT-78; HUT78#7) (RUO)

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Product Details
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BD Horizon™
TCR V beta 23; TCRBV23S1; TRBV13; TRBV23-1
Human (QC Testing)
Mouse BALB/c IgG1, κ
HUT78 T Cell Leukemia Line
Flow cytometry (Routinely Tested)
5 µl/test
AB_3685194
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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Recommended Assay Procedures

   BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

   For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant™ Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant™ Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant™ Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant™ Stain Buffer Plus (Cat. No. 566385).

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Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  5. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  8. For U.S. patents that may apply, see bd.com/patents.
  9. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  10. An isotype control should be used at the same concentration as the antibody of interest.
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569639 Rev. 1
Antibody Details
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AHUT7

The AHUT7 monoclonal antibody specifically recognizes the human variable beta 23 region of the β subunit of the human αβ T cell receptor for antigen (TCR Vβ23). The  TCR Vβ23 region is encoded by the TRBV13 gene segment. TCR Vβ23 is expressed on subsets of TCR αβ positive thymocytes and on ~0.3 to 5% of peripheral blood T cells including CD4+ and CD8+ T cells. The AHUT7 antibody is useful for multiparameter analyses designed to study the nature of TCR Vβ23-positive T cells including normal T cells as well as T cell clones, hybridomas, or tumor cell lines including H9 and HUT-78. It is also useful for analyzing TCR Vβ repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models including inflammation, autoimmunity, responses to superantigens, tumors, and infectious diseases. The AHUT7 antibody can reportedly stimulate the proliferation of TCR Vβ23-positive T cells.

569639 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
407 nm
423 nm
569639 Rev.1
Citations & References
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View product citations for antibody "569639" on CiteAb

Development References (8)

  1. Beres SB, Sylva GL, Barbian KD, et al. Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence.. Proc Natl Acad Sci U S A. 2002; 99(15):10078-83. (Biology). View Reference
  2. Ghia P, Prato G, Stella S, Scielzo C, Geuna M, Caligaris-Cappio F. Age-dependent accumulation of monoclonal CD4+CD8+ double positive T lymphocytes in the peripheral blood of the elderly.. Br J Haematol. 2007; 139(5):780-90. (Clone-specific: Flow cytometry). View Reference
  3. Giudice V, D'Addona M, Montuori N, Selleri C. The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia.. Cancers (Basel). 2021; 13(18):4513. (Clone-specific: Flow cytometry). View Reference
  4. Guilherme L, Faé KC, Oshiro SE, Tanaka AC, Pomerantzeff PM, Kalil J. T cell response in rheumatic fever: crossreactivity between streptococcal M protein peptides and heart tissue proteins.. Curr Protein Pept Sci. 2007; 8(1):39-44. (Biology). View Reference
  5. Lavoie PM, Dumont AR, McGrath H, Kernaleguen AE, Sékaly RP. Delayed expansion of a restricted T cell repertoire by low-density TCR ligands.. Int Immunol. 2005; 17(7):931-41. (Clone-specific: Flow cytometry). View Reference
  6. Pantaleo G, Soudeyns H, Demarest JF, et al. Evidence for rapid disappearance of initially expanded HIV-specific CD8+ T cell clones during primary HIV infection.. Proc Natl Acad Sci U S A. 1997; 94(18):9848-53. (Clone-specific: Fluorescence activated cell sorting). View Reference
  7. Rebai N, Pantaleo G, Demarest JF, et al. Analysis of the T-cell receptor beta-chain variable-region (V beta) repertoire in monozygotic twins discordant for human immunodeficiency virus: evidence for perturbations of specific V beta segments in CD4+ T cells of the virus-positive twins.. Proc Natl Acad Sci U S A. 1994; 91(4):1529-33. (Clone-specific: Flow cytometry). View Reference
  8. Romagné F, Kanagawa O, David-Ameline J, Peyrat MA, Bonneville M, Necker A. TCRBV23 specificity of two monoclonal antibodies revealed by a panel of human V beta chains expressed in mouse cells.. J Immunol Methods. 1995; 186(2):313-22. (Immunogen: Flow cytometry). View Reference
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569639 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.