Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Cy is a trademark of GE Healthcare.
Companion Products
The 5G6 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 10 (Siglec-10). Siglec-10 is a type I transmembrane glycoprotein encoded by SIGLEC10 that belongs to the SIGLEC (sialic acid binding Ig-like lectin) family within the immunoglobulin superfamily. The extracellular region of Siglec-10 contains five Ig-like domains including an N-terminal V-set domain that contains the sialic acid-binding site and four C2-set domains. Siglec-10 preferentially binds to glycoconjugates containing α2,3- or α2,6-linked sialic acids present on human cell surfaces and may function as an adhesion receptor. Its cytoplasmic region contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) that upon phosphorylation can bind the SH2 domains of several SH2-containing phosphatases. This CD33-related Siglec has a mouse ortholog designated Siglec-G. Siglec-10 is expressed on B cells, eosinophils, monocytes, and a subset of CD16+CD56- natural killer (NK) cells. It may play a role as an inhibitory receptor for leucocytes involved in innate and adaptive immunity. The 5G6 antibody reportedly did not crossreact with Siglecs 3, 5, 7, 8 and 9.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).
Development References (3)
-
Macauley MS, Crocker PR, Paulson JC. Siglec-mediated regulation of immune cell function in disease.. Nat Rev Immunol. 2014; 14(10):653-66. (Biology). View Reference
-
Munday J, Kerr S, Ni J, et al. Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor.. Biochem J. 2001; 355(Pt 2):489-97. (Immunogen: ELISA, Flow cytometry). View Reference
-
Pillai S, Netravali IA, Cariappa A, Mattoo H. Siglecs and immune regulation.. Annu Rev Immunol. 2012; 30:357-92. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
