Biotin Goat Anti-Rat Ig
Clone Polyclonal (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Goat Ig
- Reactivity Rat (QC Testing)
ELISA (Routinely Tested)
Immunohistochemistry-zinc-fixed, Immunohistochemistry-paraffin, Immunohistochemistry-frozen (Tested During Development)
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
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Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Immunohistochemistry: This antibody may be used as a second-step reagent for staining frozen, formalin or zinc-fixed paraffin sections. Investigators are highly encouraged to titrate the antibody for each individual application. A starting dilution range of 2.5 µg/mL - 10 µg/mL is suggested. Please note that the reactivity of polyclonal second-step antibodies to mouse or rat IgM can be reduced through adsorption against mouse or rat Ig. Because this antibody was pre-adsorbed with mouse Ig, it may display weak reactivity with some rat IgM antibodies.