Biotin Rat Anti-Mouse CD179b
Clone LM34 (RUO)
- Brand BD Pharmingen™
- Alternative Name λ5
- Concentration 0.5 mg/ml
- Isotype Rat LEW, also known as Lewis IgG2a, κ
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Purified soluble complex of recombinant µ IgH, λ5, and V[preB]
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked CD179b (μ5) and CD179a (VpreB) proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The LM34 antibody reacts with μ5 and surrogate light chain, but not VpreB alone, in transfected X63-Ag8.653 cells. It detects surrogate light chain on pro-B and pre-B cell lines, in the presence or absence of IgH, but not on IgM-positive B lymphocytes. It also detects surrogate light chain or μ5 on the surface and in the cytoplasm of pro-B or pre-B cells from the bone marrow of normal or RAG2-deficient mice. It has been noted that the (CD45R/B220) cell-surface expression of surrogate light chain is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37°C. At the earliest stages of B lymphopoiesis, before IgH is available, the surrogate light chain associates with a complex of glycoproteins, including a nonclassical cadherin, which could be involved in selective adhesion events during B-lymphocyte development. After immunization, cell-surface μ5 is detected on a subset of splenic Ig light chain-positive germinal-center B lymphocytes.
Suggested Companion Products
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Store undiluted at 4°C.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Because CD179b is expressed at very low levels on bone-marrow-derived early B lineage cells, we recommend the use of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142) and amplification of staining by the use of a "bright" second-step reagent, such as Streptavidin-PE (Cat. no. 554061) or Streptavidin-APC (Cat. no. 554067). It is difficult to distinguish the CD179b+ cells among the total bone-marrow population. Therefore, the staining protocol described by Winkler, et al., should be used. Essential features of this procedure include immunomagnetic depletion of surface IgM+ cells and incubation of the cells at 37°C for 1 hour in tissue culture medium.