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    APC Streptavidin
    Product Details
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    BD Pharmingen™
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_10050396
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Recommended Assay Procedures

    SAv-APC is a useful second-step reagent for indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric anaylsis. The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector  for fluorescence wavelengths between 640 and 680 nm.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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    554067 Rev. 12
    Antibody Details
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    This reagent is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during reagent development only or reported in the literature.

    554067 Rev. 12
    Format Details
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    APC
    Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    APC
    Red 627-640 nm
    651 nm
    660 nm
    554067 Rev.12

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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