Multicolor Flow Cytometry
Application Notes
Acquisition and Analysis
Cell Subsetting
Cytokine (Intracellular Staining) Sample Preparation
- A Comparison of the BD IntraSure™ Kit to Other BD Fixation and Permeabilization Reagetns and Invitrogen's FIX & PERM® Kit for the Detection of Intracellular Agents
- Cytokine Detection in Antigen-Activated CD8+ and CD4+ T Cells
General
- Analysis of Apoptotic Cells using the BD FACSArray™ Bioanalyzer
- An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
- BD Flow Cytometry Technical Bulletin
- Cell-Cycle Analysis Using the BD FACSArray™ Bioanalyzer
- Construction of Multicolor Antibody Panels for the Flow Cytometric Analysis of Murine Thymic Stromal Cells
- Flow Cytometry and High-Content Imaging to Identify Markers of Monocyte-Macrophage Differentiation
- Multicolor Flow Cytometric Analysis of S-phase (BrdU Incorporating)
Cells Using the BD FACSArray™ Bioanalyzer - Standardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva™ Version 6 Software
- Studying Mouse Thymocyte Development using Multiparametric Flow Cytometry: An Efficient Method to Improve an 8-Color Panel on the BD FACSVerse™ System
- Technique for Loading Cells with BD Horizon™ Violet Proliferation Dye 450 (VPD450)
Brochures
Catalogs
Experiment Sheets
Product Information Sheets
- BD APC-H7 Conjugated Antibody Reagents
- BD Biosciences Nucleic Acid Dyes
- BD™ CompBead and BD™ CompBead Plus Particles
- BD FACSelect™ Buffer Compatibility Resource
- BD Flow Cytometry Reagents: Multicolor Panels Designed for Optimal Resolution with the BD FACSVerse™ Flow Cytometer
- BD Flow Cytometry Reagents: Multicolor Panels Designed for Optimal Resolution with the BD LSRFortessa™ X-20 Cell Analyzer
- BD Horizon™ APC-R700 Reagents
- BD Horizon Brilliant™ Blue Reagents
- BD Horizon Brilliant™ Blue 515 Reagents
- BD Horizon™ Brilliant Stain Buffer
- BD Horizon Brilliant™ Ultraviolet Reagents
- BD Horizon Brilliant™ Violet 480
- BD Horizon Brilliant™ Violet Reagents
- BD Horizon Brilliant™ Violet Imaging Reagents
- BD Horizon™ Fixable Viability Stain (FVS) Reagents
- BD Horizon™ PE-CF594 Reagents
- BD Horizon™ V450 Reagents
- BD Horizon™ V500 Reagents
- BD OptiBuild™ Custom Reagents
- BD™ Optical Filters: Optimal detection of BD Horizon™ Brilliant Violet™ Polymer Conjugates
- BD PerCP-Cy™5.5 and PerCP-Conjugated Reagents
- Custom Conjugated Antibodies
- SPHERO™ Rainbow Calibration Particles
Product Lists
Protocols
Acquisition and Analysis
Apoptosis
- Annexin V with Cell Surface Antibody Staining for Suspension Cells
- Annexin V Staining of Adherent Cells for Flow Cytometry
- Annexin V Staining Protocol
- APO-BRDU™ Procedure
- APO-DIRECT™ Procedure
- Induction of Apoptosis by Treatment with Camptothecin
- Induction of Apoptosis by Treatment with Staurosporine
- Receptor-Mediated Apoptosis in Mouse Cells Using Anti-Fas/CD95 (Clone Jo2)
- Receptor-Mediated Apoptosis in Human Cells Using Anti-Fas/CD95 (Clone DX2)
Cell Cycle/DNA Analysis
- Detection of BrdU Incorporation in DNA Synthesizing Cells
- Flow Cytometry Staining Protocol for Detection of Ki-67
- Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs
- Simultaneous Measurement of Mouse Foxp3 and Ki-67 in Cultured Splenocytes
- Staining Procedure for Flow Cytometric Detection of Human Cyclins
Cytokine (Intracellular Staining) Sample Preparation
General
- Bulk Erythrocyte Lysing with Ammonium Chloride for Flow Cytometry Immunophenotyping
- Cell Surface Staining of Stem Cell Lines and Other Adherent Cells for Flow Cytometry
- Freezing and Thawing of Peripheral Blood Mononuclear Cells (PBMCs)
- Live Cell Discrimination and Measurement of Phosphorylation and Cell Surface Markers in Thawed and Activated Human PBMCs
- Tissue Preparation for Surface Antigen Staining
Human Sample Preparation
- Cell Surface Staining of Human PBMCs and Suspension Cell Lines
- Direct Immunofluorescence Staining of Mononuclear Cells
- Direct Immunofluorescence Staining of Whole Blood Using a Lyse/No-Wash Procedure
- Direct Immunofluorescence Staining of Whole Blood Using a Lyse/Wash Procedure
- Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
- Indirect Immunofluorescence Staining of Human Platelets
- Indirect Immunofluorescence Staining of Mononuclear Cells
- Isotype Control Antibody Selection Guide
- Intracellular Staining of Human Red Blood Cells
- Platelet Activation, Staining, and Analysis
- Simultaneous Measurement of Human FoxP3 and Ki-67 in Cultured PBMCs
- Surface Staining of Human Red Blood Cells
Mouse/Rat Sample Preparation
Tools
- An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
- BD Biosciences Fluorochrome/Laser Reference Chart
- BD Biosciences Fluorochrome Specifications Chart
- BD Biosciences Relative Fluorochrome Brightness Chart
- BD FACSDiva™ CS&T Bead Lot Files
- BD FACSelect™ Multicolor Panel Designer
- BD FACSuite™ CS&T Research Bead Lot Files
Webinars
- Design of Multicolor Flow Cytometry Panels Incorporating BD Horizon™ Brilliant Violet™ Dyes
- Enhancing the Performance of BD Horizon™ Brilliant Violet™ Reagents
- How to Analyze More Markers Without Adding Detectors
- Incorporating New, Bright Fluorochromes into Multicolor Panel Design
- Instrument Characterization and Performance Tracking for Digital Flow Cytometers
- Multicolor Flow Cytometry – Principles and Examples of Panel Design
- Multicolor Flow Cytometry – Principles of Panel Design
- Multicolor Flow Cytometry – Setup and Design
- Multicolor Flow Cytometry – Setup and Optimization on the BD Accuri™ C6 Flow Cytometer
- Multicolor Flow Cytometry – Setup and Optimization on the BD Accuri™ C6 Flow Cytometer (Encore Presentation)
- Optimization of Multicolor Panel Design Incorporating Low Receptor Density Antigen
BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care.
© 2019 BD. All rights reserved. Unless otherwise noted, BD, the BD Logo, and all other trademarks are property of Becton, Dickinson and Company.