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    Biotin Rat Anti-Mouse CD127
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    This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
    Biotin Rat Anti-Mouse CD127
    Expression of CD127 in the bone marrow and spleen. C57BL/6 bone marrow leukocytes (Left panels) and splenocytes (Right panels) were stained with either biotinylated rat IgG2a, κ isotype control mAb R35- 95 (Cat. no. 553928, Top panels) or biotinylated B12-1 antibody (Bottom panels) and either FITC-conjugated RA3-6B2 anti-mouse CD45R/B220, Cat. no. 553087/553088, Left panels) or FITC- conjugated 145-2C11 mAb (anti-mouse CD3e, Cat. no. 553061/553062, Right panels), followed by Streptavidin-PE (Cat. no. 554061, all panels). Flow cytometry was performed on a FACScan (BD Biosciences, San Jose, CA).
    Biotin Rat Anti-Mouse CD127
    Expression of CD127 in the bone marrow and spleen. C57BL/6 bone marrow leukocytes (Left panels) and splenocytes (Right panels) were stained with either biotinylated rat IgG2a, κ isotype control mAb R35- 95 (Cat. no. 553928, Top panels) or biotinylated B12-1 antibody (Bottom panels) and either FITC-conjugated RA3-6B2 anti-mouse CD45R/B220, Cat. no. 553087/553088, Left panels) or FITC- conjugated 145-2C11 mAb (anti-mouse CD3e, Cat. no. 553061/553062, Right panels), followed by Streptavidin-PE (Cat. no. 554061, all panels). Flow cytometry was performed on a FACScan (BD Biosciences, San Jose, CA).
    Product Details
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    BD Pharmingen™
    Interleukin-7 receptor alpha chain; IL-7R alpha; IL7RA;IL-7RA; IL-7Rα; Il7r
    Mouse (QC Testing)
    Rat WF, also known as Wistar Furth IgG2a, λ
    Mouse CD127-human IgG fusion protein
    Flow cytometry (Routinely Tested)
    0.5 mg/ml
    AB_395706
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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    555288 Rev. 8
    Antibody Details
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    B12-1

    The B12-1 monoclonal antibody specifically binds to mouse CD127 which is also known as, the Interleukin-7 Receptor alpha chain (IL-7Rα). CD127 associates with CD132 (common γ chain) to form a high-affinity signaling IL-7R complex that mediates the biological effects of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR expression upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR+ T cells.

    555288 Rev. 8
    Format Details
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    Biotin
    Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
    Biotin
    555288 Rev.8
    Citations & References
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    Development References (8)

    1. Akashi K, Kondo M, Weissman IL. Role of interleukin-7 in T-cell development from hematopoietic stem cells. Immunol Rev. 1998; 165:13-28. (Biology). View Reference
    2. Faust EA, Saffran DC, Toksoz D, Williams DA, Witte ON. Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells. J Exp Med. 1993; 177(4):915-923. (Biology). View Reference
    3. Fujihashi K, Kawabata S, Hiroi T, et al. Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes. Proc Natl Acad Sci U S A. 1996; 93(8):3613-3618. (Biology). View Reference
    4. Goodwin RG, Friend D, Ziegler SF et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell. 1990; 60(6):941-951. (Biology). View Reference
    5. Henderson AJ, Narayanan R, Collins L, Dorshkind K. Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells. J Immunol. 1992; 149(6):1973-1979. (Biology). View Reference
    6. Noguchi M, Nakamura Y, Russell SM, et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor. Science. 1993; 262(5141):1877-1880. (Immunogen). View Reference
    7. Peschon JJ, Morrissey PJ, Grabstein KH, et al. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J Exp Med. 1994; 180(5):1955-1960. (Biology). View Reference
    8. Sudo T, Nishikawa S, Ohno N, et al. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A. 1993; 90(19):9125-9129. (Biology). View Reference
    View All (8) View Less
    555288 Rev. 8

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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