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FITC Hamster Anti-Mouse CD3e
Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with APC Rat Anti-Mouse CD4 (Cat. No. 553051/561091) and APC Rat Anti-Mouse CD8a (Cat. No. 553035/561093) antibodies and either FITC Armenian Hamster IgG1, κ Isotype Control (Cat. No. 553971; Left Plot) or FITC Hamster Anti-Mouse CD3e antibody (Cat. No. 553062/553061/561827; Right Plot) at 0.5 µg/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e (or Ig Isotype Control staining) versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ FITC Hamster Anti-Mouse CD3e
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