Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
New Zealand (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-eIF-4γ
      Product Details
      Down Arrow Up Arrow


      BD Transduction Laboratories™
      Human (QC Testing), Dog (Tested in Development)
      Mouse IgG1
      Human eIF-4γ aa. 1217-1386
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
      220 kDa
      250 µg/ml
      AB_397893
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      Show More blue down arrow Show Less blue up arrow
      610536 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      40/eIF-4γ

      eIF-4 proteins are required for mRNA recognition and acceleration of translation during protein synthesis. This group of proteins consists of the RNA helicase, eIF-4A; the RNA-binding protein, eIF-4B; and the cap binding proteins, eIF-4E and eIF-4γ. eIF-4γ, also referred to as p220, is a 1396 amino acid polypeptide that migrates as a 220kDa. eIF-4γ has several potential glycosylation and phosphorylation sites that could account for its anomalous electrophoretic mobility. In addition, eIF-4γ is rapidly degraded in picornavirus-infected cells due to its content of multiple PEST sequences.

      610536 Rev. 1
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610536 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (3)

      1. Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J Biol Chem. 1996; 271(2):623-626. (Biology). View Reference
      2. Kedersha N, Chen S, Gilks N. Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules. Mol Biol Cell. 2002; 13(1):195-210. (Clone-specific: Immunofluorescence). View Reference
      3. Yan R, Rychlik W, Etchison D, Rhoads RE. Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma. J Biol Chem. 1992; 267(32):23226-23231. (Biology). View Reference
      610536 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

      Website Feedback