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R718 Mouse Anti-Human SUSD2
Product Details
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BD OptiBuild™
sushi domain-containing protein 2; Sushi domain (SCR repeat) containing; BK65A6.2; testicular tissue protein Li 190
Human (Tested in Development)
Mouse BALB/c IgG1, κ
Human retinoblastoma Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
  6. Please refer to to access safety data sheets (SDS).
  7. Please refer to for technical protocols.
  8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email:
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Antibody Details
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The W5C5 monoclonal antibody specifically reacts with SUSD2 (sushi domain containing 2), which is expressed on mesenchymal stem cells (MSC) and on some cancers and neoplastic cell lines.  It is not detected on hematopoietic cells of the peripheral blood and bone marrow. SUSD2 is a type I transmembrane protein that may interact with galectin-1 and the potential cytokine C10orf99, through which it may affect cell growth and immune responsiveness. It has a large extracellular region that contains a sushi domain (a conserved sequence that is often associated with proteins of the complement system). SUSD2 is a marker that is useful for identifying and isolating MSC from bone marrow and endometrial tissue, and those MSC may be induced to differentiate into a wide variety of cell types. Varying levels of SUSD2 expression may correlate with either suppression or enhancement of the growth of different types of neoplastic cells, presumably via a variety of direct and indirect mechanisms.

The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.

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Format Details
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The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Red 627-640 nm
695 nm
718 nm
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Citations & References
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Development References (8)

  1. Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Clone-specific). View Reference
  2. Cheng Y, Wang X, Wang P, et al. SUSD2 is frequently downregulated and functions as a tumor suppressor in RCC and lung cancer.. Tumour Biol. 2016; 37(7):9919-30. (Biology). View Reference
  3. Hultgren EM, Patrick ME, Evans RL, Stoos CT, Egland KA. SUSD2 promotes tumor-associated macrophage recruitment by increasing levels of MCP-1 in breast cancer.. PLoS ONE. 2017; 12(5):e0177089. (Biology). View Reference
  4. Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE. A novel marker of human endometrial mesenchymal stem-like cells.. Cell Transplant. 2012; 21(10):2201-14. (Clone-specific). View Reference
  5. Pan W, Cheng Y, Zhang H, et al. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest.. Sci Rep. 2014; 4:6812. (Biology). View Reference
  6. Sivasubramaniyan K, Harichandan A, Schumann S, et al. Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2.. Stem Cells Dev. 2013; 22(13):1944-54. (Immunogen). View Reference
  7. Ulrich D, Tan KS, Deane J, et al. Mesenchymal stem/stromal cells in post-menopausal endometrium.. Hum Reprod. 2014; 29(9):1895-905. (Clone-specific). View Reference
  8. Watson AP, Evans RL, Egland KA. Multiple functions of sushi domain containing 2 (SUSD2) in breast tumorigenesis.. Mol Cancer Res. 2013; 11(1):74-85. (Biology). View Reference
View All (8) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.