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Purified Rat Anti-Mouse CD31
Purified Rat Anti-Mouse CD31

Immunohistochemical staining of endothelial cells. The zinc-fixed paraffin-embedded section of U-87 MG tumor in mouse brain was stained with MEC13.3 mAb. Note the brown labeling of endothelia of blood vessels in the tumor.

Immunohistochemical staining of endothelial cells. The zinc-fixed paraffin-embedded section of U-87 MG tumor in mouse brain was stained with MEC13.3 mAb. Note the brown labeling of endothelia of blood vessels in the tumor.

Product Details
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BD Pharmingen™
EndoCAM; GPIIA; PECA1; PECAM1; Platelet endothelial cell adhesion molecule
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
129/Sv mouse-derived endothelioma cell line tEnd.1
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
15.625 µg/ml
18613
AB_393571
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunocytochemistry: The MEC13.3 antibody is recommended to test for immunohistochemical staining of Zinc-fixed paraffin sections. Tissues tested were mouse spleen, lung, heart, and thymus. Immunohistochemistry of acetone-fixed frozen sections has been reported. The antibody stains endothelial cells on small and large blood vessels. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the MEC13.3 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination of biotinylated polyclonal anti-rat Igs (multiple adsorption) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). Alternatively, the anti-rat Ig HRP Detection Kit (Cat. No. 551013) can be used to accomplish the three-step staining procedure. The clone MEC13.3 is not recommended for formalin-fixed paraffin embedded sections

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550274 Rev. 2
Antibody Details
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MEC 13.3

The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is also expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in the regulation of ligand specificity. CD38 and vitronectin receptor (αvβ3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.

550274 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550274 Rev.2
Citations & References
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Development References (7)

  1. Baldwin HS, Shen HM, Yan HC, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994; 120(9):2539-2953. (Clone-specific: Blocking). View Reference
  2. Christofidou-Solomidou M, Nakada MT, Williams J, Muller WA, DeLisser HM. Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation. J Immunol. 1997; 158(10):4872-4878. (Clone-specific: Blocking). View Reference
  3. DeLisser HM, Christofidou-Solomidou M, Strieter RM, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997; 151(3):671-677. (Clone-specific: Blocking). View Reference
  4. DeLisser HM, Newman PJ, Albelda SM. Molecular and functional aspects of PECAM-1/CD31. Immunol Today. 1994; 15(10):490-495. (Biology). View Reference
  5. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology). View Reference
  6. Vanzulli S, Gazzaniga S, Braidot MF, et al. Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell. 1997; 21(1):39-46. (Biology). View Reference
  7. Vecchi A, Garlanda C, Lampugnani MG, et al. Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol. 1994; 63(2):247-254. (Immunogen: Immunoprecipitation). View Reference
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550274 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.