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      Purified NA/LE Mouse Anti-Human CD162
      Purified NA/LE Mouse Anti-Human CD162
      Flow cytometric analysis of CD162 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified NA/LE Mouse Anti-Human CD162 (Cat. No. 556052; solid line histogram) or Purified NA/LE Mouse IgG1 κ Isotype Control (Cat. No. 554721; dashed line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristic of viable lymphocytes.
      Purified NA/LE Mouse Anti-Human CD162
      Flow cytometric analysis of CD162 expression on human peripheral blood lymphocytes. Human whole blood was stained with either Purified NA/LE Mouse Anti-Human CD162 (Cat. No. 556052; solid line histogram) or Purified NA/LE Mouse IgG1 κ Isotype Control (Cat. No. 554721; dashed line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scatter characteristic of viable lymphocytes.
      Product Details
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      BD Pharmingen™
      SELPLG; Selectin P ligand; P-selectin glycoprotein ligand 1; PSGL-1; CLA
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human PSGL-1 Transfected Cell Line
      Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation, Western blot (Reported)
      1.0 mg/ml
      6404
      AB_396323
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      556052 Rev. 6
      Antibody Details
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      KPL-1

      The KPL-1 monoclonal antibody specifically binds to p-selectin glycoprotein ligand-1 (PSGL-1), a 120-220 kDa, mucin-like type I transmembrane glycoprotein. CD162 is expressed on neutrophils, monocytes, and most lymphocytes. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). This molecule mediates rolling of leukocytes on activated endothelium and the interaction between leukocytes and activated platelets or other leukocytes found at inflammatory sites. It has also been reported that this clone completely inhibits the interaction between P-Selectin and either CD4-T cells or neutrophils.

      556052 Rev. 6
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      556052 Rev.6
      Citations & References
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      Development References (3)

      1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
      2. Snapp KR, Ding H, Atkins K, Warnke R, Luscinskas FW, Kansas GS. A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin. Blood. 1998; 91(1):154-164. (Biology: Blocking, Immunoprecipitation, Western blot). View Reference
      3. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
      556052 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

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