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      Purified Mouse Anti-Rat RT1A
      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse BALB/c IgG1, κ
      RT1B-depleted membrane glycoproteins isolated from Wistar rat spleen
      Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_395601
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      554917 Rev. 9
      Antibody Details
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      OX-18

      The OX-18 monoclonal antibody specifically recognizes a non-polymorphic determinant of rat MHC class I antigen on erythrocytes and most nucleated cells, except normal neurons and glial cells. The OX-18 antibody has been reported to block the recognition of RT1A by rat NK cells.

      This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      554917 Rev. 9
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554917 Rev.9
      Citations & References
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      Development References (4)

      1. Fukumoto T, McMaster WR, Williams AF, et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol. 1982; 12:237-243. (Immunogen: Immunoprecipitation). View Reference
      2. Kroese FGM. Immunology of the Rat. In: Pastoret PP, Griebel P, Bazin H, Govaerts A, ed. Handbook of Vertebrae Immunology. Academic Press; 1998:137-222.
      3. Mason DW, Arthur RP, Dallman MJ, Green JR, Spickett GP, Thomas ML. Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies. Immunol Rev. 1983; 74:57-82. (Immunogen: Immunohistochemistry). View Reference
      4. Neumann H, Cavalie A, Jenne DE, Wekerle H. Induction of MHC class I genes in neurons. Science. 1995; 269(5223):549-552. (Clone-specific). View Reference
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      554917 Rev. 9

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

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