Purified Mouse Anti-Mouse I-A[d]

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BD Pharmingen™ Purified Mouse Anti-Mouse I-A[d]

Name: Purified Mouse Anti-Mouse I-A[d]
Brand: BD Pharmingen™
SKU: 553545

Clone AMS-32.1

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Mouse SJL IgG2b, κ

Immunogen: BALB/c mouse splenocytes

Application: Flow cytometry (Routinely Tested), Blocking (Reported)

Concentration: 0.5 mg/ml

RRID: AB_394912

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

For immunohistochemical staining, we recommend the use of biotinylated AMS-32.1 mAb in our special formulation for immunohistochemistry, Cat. No. 550554.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Companion Products

Purified Mouse IgG2b, κ Isotype Control RUO

Size 0.5 mg Cat No. 557351

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

553545 Rev. 13

Antibody Details

AMS-32.1

The AMS-32.1 antibody specifically reacts with the I-A[d] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed.

553545 Rev. 13

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

553545 Rev.13

Citations & References

Development References (4)

Hiramine C, Nakagawa T, Hojo K. Murine nursing thymic epithelial cell lines capable of inducing thymocyte apoptosis express the self-superantigen Mls-1a. Cell Immunol. 1995; 160(1):157-162. (Clone-specific: Blocking). View Reference
Loken MR, Stall AM. Flow cytometry as an analytical and preparative tool in immunology. J Immunol Methods. 1982; 50(3):R85-R112. (Immunogen: Flow cytometry). View Reference
Ridgway WM, Ito H, Fasso M, Yu C, Fathman CG. Analysis of the role of variation of major histocompatibility complex class II expression on nonobese diabetic (NOD) peripheral T cell response. J Exp Med. 1998; 188(12):2267-2275. (Biology). View Reference
Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific). View Reference

View All (4)

553545 Rev. 13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.