Skip to main content Skip to navigation
Purified Mouse Anti-EGF Receptor
Product Details
Down Arrow Up Arrow

BD Transduction Laboratories™
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Human EGF Receptor aa. 1020-1046
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Flow cytometry (Reported), Immunohistochemistry (Not Recommended)
180 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610016 Rev. 1
Antibody Details
Down Arrow Up Arrow

Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF receptor (EGF-R) binding and activation of intrinsic tyrosine kinase activity. Following ligand binding, EGF receptor is autophosphorylated and, in turn, phosphorylates several other endogenous proteins including phospholipase Cγ. These events initiate a number of intracellular responses that include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-Myc and c-Fos.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610016 Rev. 1
Format Details
Down Arrow Up Arrow
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610016 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Deugnier MA, Faraldo MM, Janji B, Rousselle P, Thiery JP, Glukhova MA. . EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor properties.. J Cell Biol. 2002 ; 159(3):453-463. (Clone-specific: Flow cytometry). View Reference
  2. Ford MG, Valle JD, Soroka CJ, Merchant JL.. EGF receptor activation stimulates endogenous gastrin gene expression in canine G cells and human gastric cell cultures.. J Clin Invest. 1997; 99(11):2762-2771. (Clone-specific: Immunohistochemistry). View Reference
  3. Golubovskaya V, Beviglia L, Xu LH, Earp HS 3rd, Craven R, Cance W. Dual inhibition of focal adhesion kinase and epidermal growth factor receptor pathways cooperatively induces death receptor-mediated apoptosis in human breast cancer cells. J Biol Chem. 2002; 277(41):38978-38987. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Kizaka-Kondoh S, Matsuda M, Okayama H. . CrkII signals from epidermal growth factor receptor to Ras.. Proc Natl Acad Sci U S A. 1996; 93(22):12177-12182. (Clone-specific: Western blot). View Reference
  5. Ward CW, Gough KH, Rashke M, Wan SS, Tribbick G, Wang J. . Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor.. J Biol Chem. 1996; 271(10):5603-5609. (Biology). View Reference
View All (5) View Less
610016 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.