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BD Pharmingen™ PE Rat Anti-Mouse CD201
Clone 1560 (RUO)
Flow cytometric analysis of CD201 expression on bEND.3 cells. Cells from the mouse bEnd.3 (Endothelioma, ATCC CRL-2299) cell line were stained with either PE Rat IgG2b κ Isotype Control (Cat. No. 555848; dotted line histogram) or PE Rat Anti-Mouse CD201 antibody (Cat. No. 566337; solid line histogram) at 0.5 μg/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histogram showing CD201 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Flow cytometric analysis of CD201 expression on bEND.3 cells. Cells from the mouse bEnd.3 (Endothelioma, ATCC CRL-2299) cell line were stained with either PE Rat IgG2b κ Isotype Control (Cat. No. 555848; dotted line histogram) or PE Rat Anti-Mouse CD201 antibody (Cat. No. 566337; solid line histogram) at 0.5 μg/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histogram showing CD201 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
BD Pharmingen™
Procr; Epcr; APC receptor; Ccca; Centrocyclin; Ccd41
Mouse (QC Testing)
Rat IgG2b
Mouse EPCR Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739694
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Rat IgG2b, κ Isotype Control RUO
Size
100 Tests
Cat No.
555848
566337 Rev. 1
Antibody Details
1560
The 1560 monoclonal antibody specifically binds to CD201 which is also known as Endothelial protein C receptor (EPCR) or Activated protein C receptor (APC receptor). CD201 is encoded by Procr (Protein C receptor, endothelial). CD201 is a ~25 kDa single-pass type I transmembrane glycoprotein that binds both protein C and activated protein C (APC) and augments the activation of protein C by the thrombin-thrombomodulin/CD141 complex. APC functions as a serine protease that controls thrombin IIa generation by inhibiting factors Va and VIIIa. CD201 thus plays an essential role in preventing blood coagulation which may lead to tissue damage during sepsis and inflammatory responses. Procr-gene deletion reportedly leads to embryonic lethality due to placental thrombosis. CD201 is expressed by endothelial cells with higher expression on large blood vessels. The 1560 monoclonal antibody can reportedly be used for the detection of bone marrow hematopoietic stem cells (HSC) which express high levels of CD201. A soluble form of CD201 which can bind protein C and APC has also been described.
566337 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
566337 Rev.1
Citations & References
View product citations for antibody "566337" on CiteAb
Development References (5)
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Balazs AB, Fabian AJ, Esmon CT, Mulligan RC. Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow.. Blood. 2006; 107(6):2317-21. (Clone-specific: Blocking, Flow cytometry, Fluorescence activated cell sorting, Functional assay). View Reference
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Crawley JT, Gu JM, Ferrell G, Esmon CT. Distribution of endothelial cell protein C/activated protein C receptor (EPCR) during mouse embryo development.. Thromb Haemost. 2002; 88(2):259-66. (Immunogen: Immunohistochemistry). View Reference
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Disse J, Petersen HH, Larsen KS, et al. The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors.. J Biol Chem. 2011; 286(7):5756-67. (Clone-specific: Functional assay). View Reference
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Gu JM, Crawley JT, Ferrell G, et al. Disruption of the endothelial cell protein C receptor gene in mice causes placental thrombosis and early embryonic lethality.. J Biol Chem. 2002; 277(45):43335-43. (Clone-specific: Immunohistochemistry). View Reference
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Stearns-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex.. Proc Natl Acad Sci USA. 1996; 93(19):10212-6. (Biology). View Reference
566337 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.