The Zt/g4 monoclonal antibody specifically binds to RON, which is also known as CD136, Macrophage-stimulating protein receptor (MSP-Receptor, MSP-R), Macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R), or Protein-tyrosine kinase 8 (PTK8). RON is a type I transmembrane glycoprotein that belongs to the receptor tyrosine kinase family. It is expressed on cells as a disulfide-linked heterodimer. RON is expressed on macrophages, keratinocytes, erythroblasts and epithelial cells from the skin, lung, kidney, liver, intestine, colon, and bone marrow. RON reportedly regulates many physiological processes including the survival, migration, differentiation, and effector functions of a variety of cell types including epithelial cells, and macrophages. RON binds Macrophage stimulating protein (MSP). Overexpression of RON may contribute to the malignant transformation of epithelial cells.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.