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BD Horizon™ BV510 Mouse Anti-Human CD335 (NKp46)
Clone 9E2/NKp46 (also known as 9-E2) (RUO)
Two-color flow cytometric analysis of CD335 (NKp46) expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD56 antibody (Cat. No. 555518) and either BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel) or BD Horizon™ BV510 Mouse Anti-Human CD335 (NKp46) antibody (Cat. No . 564064; Right Panel). Erythrocytes were lysed with BD FACSLyse™ Lysing Buffer (Cat. No. 349202). The two-color flow cytometric dot plots show the correlated expression patterns of CD335 (NKp46) [or Ig Isotype control staining] versus CD56 for gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD335 (NKp46) expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD56 antibody (Cat. No. 555518) and either BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel) or BD Horizon™ BV510 Mouse Anti-Human CD335 (NKp46) antibody (Cat. No . 564064; Right Panel). Erythrocytes were lysed with BD FACSLyse™ Lysing Buffer (Cat. No. 349202). The two-color flow cytometric dot plots show the correlated expression patterns of CD335 (NKp46) [or Ig Isotype control staining] versus CD56 for gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD335 (NKp46) expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD56 antibody (Cat. No. 555518) and either BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel) or BD Horizon™ BV510 Mouse Anti-Human CD335 (NKp46) antibody (Cat. No . 564064; Right Panel). Erythrocytes were lysed with BD FACSLyse™ Lysing Buffer (Cat. No. 349202). The two-color flow cytometric dot plots show the correlated expression patterns of CD335 (NKp46) [or Ig Isotype control staining] versus CD56 for gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Two-color flow cytometric analysis of CD335 (NKp46) expression on human peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD56 antibody (Cat. No. 555518) and either BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; Left Panel) or BD Horizon™ BV510 Mouse Anti-Human CD335 (NKp46) antibody (Cat. No . 564064; Right Panel). Erythrocytes were lysed with BD FACSLyse™ Lysing Buffer (Cat. No. 349202). The two-color flow cytometric dot plots show the correlated expression patterns of CD335 (NKp46) [or Ig Isotype control staining] versus CD56 for gated events with the forward and side light-scatter characteristics of intact peripheral blood lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
NCR1; NK-p46; hNKp46; LY94; Natural cytotoxicity triggering receptor 1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human NKp46 Recombinant Protein
Flow cytometry (Routinely Tested)
5 µl
VIII 80442
AB_2738571
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Brilliant Violet™ 510 is a trademark of Sirigen.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV510 Mouse IgG1, k Isotype Control RUO
Size
50 µg
Cat No.
562946
APC Mouse Anti-Human CD56 (NCAM-1) RUO
Size
100 Tests
Cat No.
555518
564064 Rev. 1
Antibody Details
9E2/NKp46
The 9E2/Nkp46 monoclonal antibody specifically binds to CD335. CD335 is also known as the Natural killer cell p46-related protein (NKp46) and the Natural cytotoxicity triggering receptor 1 (NCR1). CD335 is a 46 kDa type I membrane glycoprotein that is expressed on resting and activated NK cells. Its extracellular region contains two C2-type, Ig-like domains. The transmembrane domain contains a positively charged amino acid (Arg) which could be involved in stabilizing the association with CD3ζ. Its intracellular region does not contain immunoreceptor tyrosine-based activating motifs (ITAM), but it is linked to intracytoplasmic transduction machinery by its association with CD3ζ and FcεRIγ adaptor proteins. CD335 along with NKp30 and NKp44 are referred to as natural cytotoxicity receptors (NCR). These receptors play very important roles in cells that kill virus-infected target cells, tumor cells and MHC-class I-unprotected cells.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.
564064 Rev. 1
Format Details
BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV510
327 nm, 405 nm
512 nm
564064 Rev.1
Citations & References
View product citations for antibody "564064" on CiteAb
Development References (4)
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Mandelboim O, Porgador A. NKp46. Int J Biochem Cell Biol. 2001; 33(12):1147-1150. (Biology). View Reference
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Nakajima H, Cella M, Bouchon A, et al. Patients with X-linked lymphoproliferative disease have a defect in 2B4 receptor-mediated NK cell cytotoxicity. Eur J Immunol. 2000; 30(11):3309-3318. (Immunogen: Flow cytometry, Functional assay). View Reference
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Sivori S, Pende D, Bottino C, et al. NKp46 is the major triggering receptor involved in the natural cytotoxicity of fresh or cultured human NK cells. Correlation between surface density of NKp46 and natural cytotoxicity against autologous, allogeneic or xenogeneic target cells. Eur J Immunol. 1999; 29(5):1656-1666. (Biology). View Reference
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Sivori S, Vitale M, Morelli L, et al. p46, a novel natural killer cell-specific surface molecule that mediates cell activation. J Exp Med. 1997; 186(7):1129-1136. (Biology). View Reference
564064 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.