Preparation And Storage
Recommended Assay Procedures
1. ELISA Detection: The biotinylated 359-81-11 antibody (Cat. No. 554555) is useful as a detection antibody for a sandwich ELISA for measuring human LT-α protein levels. Biotinylated 359-81-11 antibody can be paired with the purified 359-238-8 antibody (Cat. No. 551222) as the capture antibody, with recombinant LT-α (Cat. No. 554619) as the standard. Biotinylated 359-81-11 antibody should be titrated (0.5 - 2.0 µg/ml) to determine optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of human LT-α ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring LT-α in serum or plasma our LT-α BD OptEIA™ set (Cat. No. 550995) is specially formulated and recommended.
2. Neutralization: The NA/LE™ 359-81-11 antibody (Cat. No. 554553) has been reported to be useful for neutralization of human LT-α bioactivity. A suitable NA/LE mouse IgG1 isotype control to match the 359-81-11 antibody is the 107.3 antibody, (Cat. No. 554721).
3. IF/Flow: The 359-81-11 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate LT-α producing cells within mixed cell populations. The PE-conjugated 359-81-11 antibody (Cat. No. 554556) is especially suitable for these studies.
4. Western Blot: The 359-81-11 antibody is not recommended for Western blotting.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 359-81-11 antibody reacts with human Lymphotoxin-α (LT-α, also known as tumor necrosis factor-β or TNF-β). The immunogen used to generate the 359-81-11 hybridoma was recombinant human LT-α. This is a neutralizing antibody.
Development References (4)
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Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
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Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Methodology). View Reference
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Meager A, Parti S, Leung H, Peil E, Mahon B. Preparation and characterization of monoclonal antibodies directed against antigenic determinants of recombinant human tumour necrosis factor (rTNF). Hybridoma. 1987; 6(3):305-312. (Clone-specific: ELISA, Neutralization). View Reference
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Meager A, Parti S, Leung H, et al. A two-site sandwich immunoradiometric assay of human lymphotoxin with monoclonal antibodies and its applications. J Immunol Methods. 1987; 104(2):31-42. (Clone-specific: ELISA, Neutralization). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.