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      Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III
      Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III
      Flow cytometry analysis of Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III in H9 cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) were differentiated into Neural Precursor cells (NPCs) and grown for 4 passages before differentiating into neurons and glia for 12 days. The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 20 minutes at room temperature, permeabilized with BD Phosflow™ Perm Buffer I (Cat. No. 557885), and then stained with either Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III (solid line) or Alexa Fluor® 488 Mouse IgG2a, κ Isotype control (Cat. No. 558055, dashed line).  This antibody also works in BD Phosflow™ Perm Buffer III (Cat. No. 558050). Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
      Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III
      Flow cytometry analysis of Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III in H9 cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) were differentiated into Neural Precursor cells (NPCs) and grown for 4 passages before differentiating into neurons and glia for 12 days. The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 20 minutes at room temperature, permeabilized with BD Phosflow™ Perm Buffer I (Cat. No. 557885), and then stained with either Alexa Fluor® 488 Mouse anti-β-Tubulin, Class III (solid line) or Alexa Fluor® 488 Mouse IgG2a, κ Isotype control (Cat. No. 558055, dashed line).  This antibody also works in BD Phosflow™ Perm Buffer III (Cat. No. 558050). Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.
      Product Details
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      BD Pharmingen™
      tubulin, beta 3; MC1R; TUBB3; TUBB4; tubulin, beta-4
      Human (QC Testing), Rat (Reported)
      Mouse IgG2a
      Rat brain microtubules
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_1645344
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
      4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
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      560381 Rev. 2
      Antibody Details
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      TUJ1

      Microtubules are formed by the self assembly of tubulin and are one of the major components of the eukaryotic cytoskeleton. The two main tubulin isoforms, α - and ß-tubulin, are usually products of separate genes. The ß-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III ß-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III ß-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.

      560381 Rev. 2
      Format Details
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      Alexa Fluor™ 488
      Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 488
      Blue 488 nm
      494 nm
      517 nm
      560381 Rev.2
      Citations & References
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      Development References (3)

      1. Geisert EE Jr, Frankfurter A. The neuronal response to injury as visualized by immunostaining of class III beta-tubulin in the rat. Neurosci Lett. 1989; 102(2-3):137-141. (Biology: Immunohistochemistry). View Reference
      2. Katsetos CD, Del Valle L, Geddes JF et al. Aberrant localization of the neuronal class III beta-tubulin in astrocytomas. Arch Pathol Lab Med. 2001; 125(5):613-624. (Biology: Immunocytochemistry (cytospins)). View Reference
      3. Mozzetti S, Ferlini C, Concolino P et al. Class III beta-tubulin overexpression is a prominent mechanism of paclitaxel resistance in ovarian cancer patients. Clin Cancer Res. 2005; 11(1):298-305. (Biology: Immunohistochemistry). View Reference
      560381 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

      Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

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