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PE Mouse Anti-Human NPM-ALK/ALK Set

PE Mouse Anti-Human NPM-ALK/ALK Set

(RUO)
Product Details
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BD Pharmingen™
Human (QC Testing)
Intracellular staining (flow cytometry) (Routinely Tested)
RUO
AB_397208


Description

Reacts with ALK-NPM, a chimeric gene product of approximately 80 kDa, which is the result of t(2;5)(p23;q35) chromosomal translocation. The gene at 5q35 encodes for the cell cycle-regulated nucleolar protein, nucleophism (NPM), while the gene at 2p23 encodes a novel receptor tyrosine kinase, anaplastic lymphoma kinase (ALK). The 2;5 translocation results in the expression of NPM-ALK protein, or p80, comprised of the N-terminal portion of NPM (about 40% of the molecule) linked to the entire intracellular portion of ALK. ALK1 antibody recognizes a formalin resistant epitope in both the NPM-ALK chimeric and the 200 kDa normal human ALK proteins. Cytoplasmic and nuclear expression of NPM-ALK is seen in t(2;5)+ cell lines Su-DHL-1 and Karpas 299.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

We recommend to use the Cytofix/cytoperm solution (Cat. No. 554714) for the cell fixation and permeabilization. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.

Product Notices

  1. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
559257 Rev. 8
Components
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Description Quantity/Size Part Number EntrezGene ID
PE Mouse Anti-Human NPM-ALK/ALK 100 Tests (1 ea) 51-37595X N/A
PE Mouse IgG3 Kappa Isotype Control 100 Tests (1 ea) 51-35435X N/A
559257 Rev. 8
Citations & References
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Development References (3)

  1. Morris SW, Kirstein MN, Valentine MB, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma. Science. 1994; 263(5151):1281-1284. (Biology). View Reference
  2. Pittaluga S, Wlodarska I, Pulford K, et al. The monoclonal antibody ALK1 identifies a distinct morphological subtype of anaplastic large cell lymphoma associated with 2p23/ALK rearrangements. Am J Pathol. 1997; 151(2):343-351. (Biology). View Reference
  3. Pulford K, Lamant L, Morris SW, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood. 1997; 89(4):1394-1404. (Biology). View Reference
559257 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.