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PE Mouse Anti-Human CD9
Product Details
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BD™
CD9 antigen (p24); 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; MRP-1; TSPAN29
Human
Mouse BALB/c IgG1, κ
Human C-ALL Cells
Flow cytometry
25 µg/mL
20 μL
III 617
928
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

341647 Rev. 1
Antibody Details
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M-L13

The CD9 antibody, clone M-L13, is derived from the hybridization of mouse myeloma cells with spleen cells isolated from mice immunized with common acute lymphoblastic leukemia (cALL) cells.

The CD9 antibody recognizes a 24-kilodalton (kDa) cell surface glycoprotein belonging to the tetraspanin family. The CD9 antigen is also known as p24, tetraspanin-29 (Tspan-29), motility-related protein-1 (MRP-1), and leucocyte antigen MIC3.

341647 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
341647 Rev.1
Citations & References
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View product citations for antibody "341647" on CiteAb

Development References (18)

  1. Aoyama K, Oritani K, Yokota T, et al. Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood. 1999; 93:2586-2594. (Biology).
  2. Berditchevski F, Odintsova E. Characterization of integrin-tetraspanin adhesion complexes: role of tetraspanins in integrin signaling. J Biol Chem. 1999; 146:477-492. (Biology).
  3. Boucheix C, Benoit P, Krief P, et al. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:780-782.
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Johnson GD, MacLennan ICM. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:187-189.
  7. Maecker HT, Todd SC, Levy S. The tetraspanin superfamily: molecular facilitators. FASEB J. 1997; 11:428-442. (Biology).
  8. Ozaki Y, Satoh K, Kuroda K, et al. Anti-CD9 monoclonal antibody activates p72syk in human platelets. J Biol Chem. 1995; 270:15119-15124. (Biology).
  9. Powling MJ, Cox AD, Higgins J, Hardisty RM, Goodall AH. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1012-1014.
  10. Slaper-Cortenbach ICM, Admiraal LG, von dem Borne AEG, van Leeuwen EF, Tetteroo PAT. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:480-484.
  11. Tai XG, Toyooka K, Yashiro Y, et al. CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis. J Immunol. 1997; 159:3799-3807. (Biology).
  12. Weir EG, Cowan K, LeBeau P, Borowitz MJ. A limited antibody panel can distinguish B-precursor acute lymphoblastic leukemia from normal B precursors with four color flow cytometry: implications for residual disease detection. Leukemia. 1999; 13:558-567. (Biology).
  13. Zeleznik NJ, Hollingsworth MA, Metzgar RS. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:389-392.
  14. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
  15. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference
  16. von dem Borne AEG, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:989-990.
  17. von dem Borne AEGKr, Admiraal LG, Daams M, Hogervorst F, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:967-969.
  18. von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
View All (18) View Less
341647 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.