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PE Mouse Anti-Human CD69
Product Details
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BD™
AIM; CLEC2C; EA1; GP32/28; Leu-23; MLR-3; VEA; BL-AC/P26
Human
Mouse BALB/c IgG1, κ
CD8+ alloantigen-directed CTL cell line
Flow cytometry
3.0 μg/mL
20 μL
969
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 100 μg in 2.0 mL (50 μg/mL) of PBS. The PE conjugate is supplied as 3.0 μg in 1.0 mL (3.0 μg/mL) of PBS. The PerCP conjugate is supplied as 50 μg in 1.0 mL (50 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. The APC conjugate is supplied as 100 μg in 0.5 mL (200 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341652 Rev. 1
Antibody Details
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L78

The CD69 antibody, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen–directed cytotoxic T-lymphocyte (CTL) cell line.

The CD69 antibody recognizes a very early human activation antigen that is a disulfide-bonded homodimer consisting of Mr 60-kilodalton (kDa) polypeptides with one or two N-linked oligosaccharides.

341652 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
341652 Rev.1
Citations & References
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View product citations for antibody "341652" on CiteAb

Development References (10)

  1. Borrego F, Robertson MJ, Ritz J, Peña J, Solana R. CD69 is a stimulatory receptor for natural killer cell and its cytotoxic effect is blocked by CD94 inhibitory receptor.. Immunology. 1999; 97(1):159-65. (Biology). View Reference
  2. Chen JH, Prince H, Buck D, et al. Leu-23: an early activation antigen on human lymphocytes. Fed Proc. 1988; 2:A1214. (Biology).
  3. Graber M, Bockenstedt LK, Weiss A. Signaling via the inositol phospholipid pathway by T cell antigen receptor is limited by receptor number.. J Immunol. 1991; 146(9):2935-43. (Biology). View Reference
  4. Lanier LL, Buck DW, Rhodes L, et al. Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen.. J Exp Med. 1988; 167(5):1572-85. (Biology). View Reference
  5. Pisegna S, Zingoni A, Pirozzi G, et al. Src-dependent Syk activation controls CD69-mediated signaling and function on human NK cells.. J Immunol. 2002; 169(1):68-74. (Biology). View Reference
  6. Schwarting R, Biedobitek G, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:428-432.
  7. Testi R, Philips J, Lanier LL. Constitutive expression of a phosphorylated activation (Leu-23) by CD3 bright thymocytes. J Immunol. 1988; 141:2257. (Biology).
  8. Testi R, Philips JH, Lanier LL. Leu-23 induction as an early marker for functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular (Ca ++ ), and stimulation of protein kinase C. J Immunol. 1988; 2:1214. (Biology).
  9. Testi R, Phillips JH, Lanier LL. T cell activation via Leu-23 (CD69).. J Immunol. 1989; 143(4):1123-8. (Biology). View Reference
  10. Testi R, Pulcinelli F, Frati L, Gazzaniga PP, Santoni A. CD69 is expressed on platelets and mediates platelet activation and aggregation.. J Exp Med. 1990; 172(3):701-7. (Biology). View Reference
View All (10) View Less
341652 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.