CD274 (MIH1) PE-Cy7

BD® CD274 (MIH1) PE-Cy7

Name: CD274 (MIH1) PE-Cy7
Brand: BD®
SKU: 665123

Clone MIH1

(ASR)

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Scientific Resources

Product Details

Brand: BD®

Alternative Name: B7-H1; B7-H; PD-L1; PDL1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human CD274 Transfected Cell Line

Application: Flow cytometry

Workshop Number: VIII 80536

Entrez Gene ID: 29126

Regulatory Status: ASR

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Antibody Details

MIH1

The CD274 antibody specifically binds to the CD274 antigen, also known as B7homolog (B7-H1), programmed cell death 1 ligand 1 (PDCD 1 ligand, PDCD1L1, PDCD1PG1), or programmed death ligand 1 (PD-L1, PDL1). CD274 and CD273 (programmed death ligand 2) are type 1 transmembrane glycoproteins that belong to the B7 family and serve as ligands for CD279 (Program Death 1/PD-1).

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Format Details

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE-Cy7

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

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Citations & References

Development References (2)

Youngnak P, Kozono Y, Kozono H, et al. Differential binding properties of B7-H1 and B7-DC to programmed death-1. Biochem Biophys Res Commun. 2003; 307(3):672-677. View Reference
Youngnak-Piboonratanakit P, Tsushima F, Otsuki N, et al. The expression of B7-H1 on keratinocytes in chronic inflammatory mucocutaneous disease and its regulatory role. Immunol Lett. 2004; 94(3):215-222. View Reference

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.