Skip to main content Skip to navigation
CD23 APC-R700
Product Details
Down Arrow Up Arrow


BD Horizon™
CD23A; Fc-epsilon-RII; FCER2; FCE2; IGEBF; BLAST-2; CLEC4J; Leu-20
Human
Mouse BALB.B IgG1, κ
Epstein-Barr Virus Transformed Cell Line
Flow cytometry
12.5 µg/mL
5 μL
2208
Phosphate buffered saline (PBS) with BSA and ProClin.
ASR


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

659116 Rev. 1
Antibody Details
Down Arrow Up Arrow
EBVCS-5

The CD23 antibody, clone EBVCS-5, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with an in vitro–transformed EBV cell line.

The CD23 antibody recognizes a 45-kilodalton (kDa) type II membrane glycoprotein, which is a human B-lymphocyte differentiation antigen. The CD23 antigen is also known as the low affinity IgE receptor, Fc epsilon RII, and FcεRII.

659116 Rev. 1
Format Details
Down Arrow Up Arrow
APC-R700
The BD Horizon™ APC-R700 (APC-R700) Dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of an Allophycocyanin (APC) dye donor that has excitation maximum (Ex Max) of 651-nm and an acceptor dye, R700, with an emission maximum (Em Max) at 706-nm. APC-R700, driven by BD innovation, is designed to be excited by the red (627–640-nm) laser and detected using an optical filter centered near 710-nm (e.g., a 720/40-nm bandpass filter). APC-R700 is a brighter alternative to Alexa Fluor™ 700. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC-R700
Red 627-640 nm
651 nm
706 nm
659116 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "659116" on CiteAb

Development References (12)

  1. Capron M, Jouault T, Prin L, et al. Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages.. J Exp Med. 1986; 164(1):72-89. (Biology). View Reference
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Clinical and Laboratory Standards Institute. 2005. (Biology).
  4. Gordon J, Rowe M, Walker L, Guy G. Ligation of the CD23,p45 (Blast-2, EBVCS) antigen triggers the cell-cycle progression of activated B lymphocytes. Eur J Immunol. 1986; 16:1075-1080. (Biology).
  5. Gordon J, Webb AJ, Walker L, Guy GR, Rowe M. Evidence of an association between CD23 and the receptor for a low molecular weight B cell growth factor. Eur J Immunol. 1986; 16:1627-1630. (Biology).
  6. Kikutani H, Inui S, Sato R, et al. Molecular structure of human lymphocyte receptor for immunoglobulin E.. Cell. 1986; 47(5):657-65. (Biology). View Reference
  7. Kikutani H, Suemura M, Owaki H, et al. Fc epsilon receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching.. J Exp Med. 1986; 164(5):1455-69. (Biology). View Reference
  8. Kintner C, Sugden B. Identification of antigenic determinants unique to the surfaces of cells transformed by Epstein-Barr Virus. Nature. 1981; 294:458-460. (Biology).
  9. Nadler LM. B Cell/Leukemia Panel Workshop: summary and comments. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, ed. Leukocyte Typing II: Human B Lymphocytes. New York: Springer-Verlag; 1986:3-43.
  10. Sarfati M, Ishihara H, Delespesse G. CD23 Workshop Panel report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:530-533.
  11. Thorley-Lawson D, Nadler L, Bhan A, Schooley R. Blast-2 (EBVCS), an early cell surface marker of human B cell activation, is superinduced by Epstein Barr Virus. J Immunol. 1985; 134:3007-3012. (Biology).
  12. Yukawa K, Kikutani H, Owaki H, et al. A B-cell– specific differentiation antigen, CD23, is a receptor for IgE (FcεR) on lymphocytes. J Immunol. 1987; 138:2576-2580. (Biology).
View All (12) View Less
659116 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.