APC-H7 Mouse Anti-Human CD24

BD™ APC-H7 Mouse Anti-Human CD24

Name: APC-H7 Mouse Anti-Human CD24
Brand: BD™
SKU: 658331

Clone ML5

(RUO)

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Product Details

Brand: BD™

Alternative Name: Heat Stable Antigen Homologue (HSA); Ba-1; CD24A

Reactivity: Human

Isotype: Mouse IgG2a, κ

Application: Flow cytometry

Concentration: 200 μg/mL

Vol. Per Test: 5 μL

Workshop Number: IV B243; V CD24.5

Entrez Gene ID: 100133941

Regulatory Status: RUO

658331 Rev. 1

Antibody Details

ML5

The CD24 antibody, clone ML5, is derived from the hybridization of mouse myeloma cells with spleen cells from immunized mice.

The CD24 antibody reacts specifically with a 35–45 kilodalton (kDa) highly glycosylated sialoprotein anchored to the cell surface by glycosylphosphatidylinositol (GPI). The CD24 antigen is also known as heat stable antigen (HSA) homologue, BA-1, and small cell lung carcinoma cluster-4 antigen.

658331 Rev. 1

Format Details

APC-H7

The BD Horizon™ APC-H7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 659 nm and an acceptor dye, H7, with an emission maximum (Em Max) at 782 nm. APC-H7, driven by BD innovation, is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: APC-H7

Excitation Source: Red 627-640 nm

Excitation Max: 659 nm

Emission Max: 782 nm

658331 Rev.1

Citations & References

View product citations for antibody "658331" on CiteAb

Development References (26)

Barclay AN, Brown MH, Law SKA, McKnight AJ, Tomlinson MG, van der Merwe PA. The Leucocyte Antigen FactsBook 2nd ed. San Diego: Academic Press; 1997.
Barclay AN, Brown MH, Law SKA, McKnight AJ, Tomlinson MG, van der Merwe PA. The Leucocyte Antigen FactsBook 2nd ed. San Diego: Academic Press; 1997.
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Fischer GF, Majdic O, Gadd S, Knapp W. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. 1990; 144:638-641. (Biology).
Fischer GF, Majdic O, Gadd S, Knapp W. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. 1990; 144:638-641. (Biology).
Kay R, Rosten PM, Humphries RK. CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol. 1991; 147:1412-1416. (Biology).
Kay R, Rosten PM, Humphries RK. CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol. 1991; 147:1412-1416. (Biology).
Le Bien TW. CD24 Workshop Panel Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:539-541.
Le Bien TW. CD24 Workshop Panel Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:539-541.
Lund-Johansen F, Olweus J, Symington FW, et al. Activation of human monocytes and granulocytes by monoclonal antibodies to glycosylphosphatidylinositol-anchored antigens. Eur J Immunol. 1993; 23:2782-2791. (Biology).
Lund-Johansen F, Olweus J, Symington FW, et al. Activation of human monocytes and granulocytes by monoclonal antibodies to glycosylphosphatidylinositol-anchored antigens. Eur J Immunol. 1993; 23:2782-2791. (Biology).
Petrasch S, Perez-Alvarez C, Schmitz J, Kosco M, Brittinger G. Antigenic phenotyping of human follicular dendritic cells isolated from nonmalignant and malignant lymphatic tissue. Eur J Immunol. 1990; 20:1013-1018. (Biology).
Petrasch S, Perez-Alvarez C, Schmitz J, Kosco M, Brittinger G. Antigenic phenotyping of human follicular dendritic cells isolated from nonmalignant and malignant lymphatic tissue. Eur J Immunol. 1990; 20:1013-1018. (Biology).
Rabinovitch PS, Clark EA, Pezzutto A, Ledbetter JA, Draves KE. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:435-439.
Rabinovitch PS, Clark EA, Pezzutto A, Ledbetter JA, Draves KE. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:435-439.
Weber E, Lehmann HP, Beck-Sickinger AG, et al. Antibodies to the protein core of the small cell lung cancer workshop antigen cluster-w4 and to the leucocyte workshop antigen CD24 recognize the same short protein sequence leucine-alanine-proline. Clin Exp Immunol. 1993; 93:279-285. (Biology).
Weber E, Lehmann HP, Beck-Sickinger AG, et al. Antibodies to the protein core of the small cell lung cancer workshop antigen cluster-w4 and to the leucocyte workshop antigen CD24 recognize the same short protein sequence leucine-alanine-proline. Clin Exp Immunol. 1993; 93:279-285. (Biology).
Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
de Rie MA, Terpstra FG, van Lier RAW, Von dem Borne AEG, Miedema F. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:402-405.
de Rie MA, Terpstra FG, van Lier RAW, Von dem Borne AEG, Miedema F. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:402-405.
van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference
van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference

View All (26)

658331 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.