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Anti-bcl-2 FITC
Product Details
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BD™
BCL2; Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2
Human
Mouse BALB/c IgG1, κ
Human Bcl-2 synthetic peptide aa. 41-54
Flow cytometry
1.0 μg/mL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
ASR


Preparation And Storage

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340650 Rev. 1
Antibody Details
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Bcl-2/100

The Anti–bcl-2 antibody, clone 100, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the bcl-2α peptide (amino acids 41-54).

The Anti–bcl-2 antibody recognizes a 26-kilodalton (kDa) protein encoded by the 230 kb bcl-2 protooncogene. The protein is a regulator of the apoptotic process, and elevated levels can provide resistance to cell death. The bcl-2 protein is expressed in the interior of cells, frequently localized to the mitochondrial membrane.

340650 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
340650 Rev.1
Citations & References
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View product citations for antibody "340650" on CiteAb

Development References (11)

  1. Aiello A, Delia D, Borrello MG, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992; 13(5):502-509. (Biology). View Reference
  2. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  3. Cleary ML, Smith SD, Sklar J. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 1986; 47:19-28. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Coustan-Smith E, Kitanaka A, Pui C-H, et al. Clinical relevance of bcl-2 overexpression in childhood acute lymphoblastic leukemia. Blood. 1996; 87:1140-1146. (Biology).
  6. DiGiuseppe JA, LeBeau P, Augenbraun J, Borowitz MJ. Multiparameter flow-cytometric analysis of bcl-2 and fas expression in normal and neoplastic hematopoiesis. Am J Clin Pathol. 1996; 106:345-351. (Biology).
  7. Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC. Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res. 1993; 53(19):4701-4714. (Biology). View Reference
  8. Pezzella F, Tse AG, Cordell JL, Pulford KA, Gatter KC, Mason DY. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol. 1990; 137(2):225-232. (Biology). View Reference
  9. Reed JC. Double identity for proteins of the bcl-2 family. Nature. 1997; 387:773-776. (Biology).
  10. Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A. 1986; 83(14):5214-5218. (Biology). View Reference
  11. Zamai L, Canonico B, Gritzapis A, et al. Intracellular detection of Bcl-2 and p53 proteins by flow cytometry: comparison of monoclonal antibodies and sample preparation protocols. J Biol Regul Homeost Agents. 2002; 16:289–302. (Biology).
View All (11) View Less
340650 Rev. 1

 

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

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