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PE Rat Anti-Mouse PDC-TREM
Multi-color flow cytometric analysis of PDC-TREM expression on mouse plasmacytoid dentritic cells (pDCs). Splenocytes from BALB/c mice were cultured in the presence of CpG-A (Invivogen) for 24 hours. The cells were then washed, and Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142) was added. The cells were stained simultaneously with optimal concentrations of FITC Hamster Anti-Mouse CD3e (Cat. No. 553061, 553062, or 561827), FITC Rat anti-Mouse CD19 (Cat. No. 553785, 557398, or 561740), APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092 or 561880), and BD Horizon™ BUV395 Hamster Anti-Mouse CD11c (Cat. No. 564080), plus either PE Rat IgG2a, κ Isotype Control (Cat.No. 553930; dashed line histogram) or PE Rat Anti-Mouse PDC-TREM (Cat. No. 566926; solid line histogram) at 0.5 µg/test. The left plot was derived from CD3e-negative, CD19-negative viable leukocytes and displays the gating of the CD45R/B220-positive, CD11c-positive pDC population. The right plot shows the expression of PDC-TREM (or Ig Isotype Control staining) on the gated pDCs. Flow cytometry and data analysis was performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Rat Anti-Mouse PDC-TREM
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