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FITC Rat anti-Mouse Ly-6G
FITC Rat anti-Mouse Ly-6G

Flow cytometric analysis of expression of Ly-6G on bone-marrow leukocytes. BALB/c bone-marrow cell suspensions were stained with either FITC Rat Anti-Mouse Ly-6G (Cat. No. 551460/561105, dashed line histograms) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929, shaded histograms), in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with propidium iodide, and leukocyte subsets were distinguished by their light scatter profiles. Left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™.

Flow cytometric analysis of expression of Ly-6G on bone-marrow leukocytes. BALB/c bone-marrow cell suspensions were stained with either FITC Rat Anti-Mouse Ly-6G (Cat. No. 551460/561105, dashed line histograms) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929, shaded histograms), in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), (Cat. No. 553141/553142). Non-viable leukocytes were excluded by staining with propidium iodide, and leukocyte subsets were distinguished by their light scatter profiles. Left panel displays lymphoid and erythroid cells, and the right panel displays myeloid cells. Flow cytometry was performed on a FACSCalibur™.

Product Details
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BD Pharmingen™
Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Ly-6G-transfected EL4J Cell Line
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394207
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Recommended Assay Procedures

For flow cytometry of leukocyte suspensions, we recommend the use of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™), (Cat. No. 553141/553142).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Antibody Details
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1A8

The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
Citations & References
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Development References (1)

  1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen). View Reference

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.