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    BUV395 Rat Anti-Mouse IgM
    BUV395 Rat Anti-Mouse IgM
    Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e (Cat. No. 562286/562332) and either BUV395 Rat IgG2a, κ Isotype Control (Cat. No. 563556, Left Panel) or BUV395 Rat Anti-Mouse IgM antibody (Cat. No. 564025, Right Panel). Fluorescence two-color dot plots showing the correlated expression of IgM (or Ig Isotype control staining) versus CD3e were derived from gated events with the light-scattering characteristics of viable splenocytes. Flow cytometric analysis was performed on a BD™ LSR II.
    BUV395 Rat Anti-Mouse IgM
    Two-color flow cytometric analysis of IgM expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE-CF594 Hamster Anti-Mouse CD3e (Cat. No. 562286/562332) and either BUV395 Rat IgG2a, κ Isotype Control (Cat. No. 563556, Left Panel) or BUV395 Rat Anti-Mouse IgM antibody (Cat. No. 564025, Right Panel). Fluorescence two-color dot plots showing the correlated expression of IgM (or Ig Isotype control staining) versus CD3e were derived from gated events with the light-scattering characteristics of viable splenocytes. Flow cytometric analysis was performed on a BD™ LSR II.
    Product Details
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    BD Horizon™
    Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
    Pooled Mouse Ig
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_2738550
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimum conditions, and unconjugated antibody and free BD Horizon BUV395 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the Brilliant Stain Buffer (Cat. No. 563794/566349) or the Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
    7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
    8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    566217 Rev. 1
    Antibody Details
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    R6-60.2

    The R6-60.2 antibody monoclonal antibody specifically binds to mouse Immunoglobulin M (IgM) of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. The R6-60.2 antibody has not been shown to stimulate B-cell proliferation.

    The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355 nm laser equipped instrument. With an Ex Max at 348 nm  and an Em Max at 395 nm, this dye has virtually no spillover into any other detector. BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

    566217 Rev. 1
    Format Details
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    BUV395
    The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BUV395
    Ultraviolet 355 nm
    348 nm
    395 nm
    566217 Rev.1
    Citations & References
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    Development References (3)

    1. BD Biosciences Pharmingen. Unpublished results. .
    2. Gavin AL, Duong B, Skog P, et al. δBAFF, a splice isoform of BAFF, opposes full-length BAFF activity in vivo in transgenic mouse models. J Immunol. 2005; 75(1):319-328. (Clone-specific: ELISA). View Reference
    3. Touma M, Keskin DB, Shiroki F, et al. Impaired B cell development and function in the absence of IκBNS. J Immunol. 2011; 187(8):3942-3952. (Clone-specific: Flow cytometry). View Reference
    566217 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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