This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected.
Note: This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative.
For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.