Multicolor flow cytometric analysis of Vβ 13 TCR expression on mouse lymph node T cells. C57BL/6 mouse lymph node cells were stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047) and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 550617, left panel) or a PE Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 561541, right panel). Two-color flow cytometric dot plots showing the expression of Vβ 13 TCR (or Ig Isotype Control staining) versus CD4 and CD8 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
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Multicolor flow cytometric analysis of Vβ 13 TCR expression on mouse lymph node T cells. C57BL/6 mouse lymph node cells were stained with FITC Rat Anti-Mouse CD4 (Cat. No. 553046/553047) and FITC Rat Anti-Mouse CD8a (Cat. No. 553030/553031) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 550617, left panel) or a PE Mouse Anti-Mouse Vβ 13 TCR (Cat. No. 561541, right panel). Two-color flow cytometric dot plots showing the expression of Vβ 13 TCR (or Ig Isotype Control staining) versus CD4 and CD8 were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD™ LSR II.
Product Details
BD Pharmingen™
TCR V beta 13; TCR Vβ 13; TCR Vβ13; Vβ 13 T-cell Receptor
Mouse (QC Testing)
Mouse C57BR IgG1, κ
Not reported
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10898187
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
550617
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FITC Rat Anti-Mouse CD4 RUO
Size
0.1 mg
Cat No.
553046
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FITC Rat Anti-Mouse CD4 RUO
Size
0.5 mg
Cat No.
553047
FITC Rat Anti-Mouse CD8a RUO
Size
0.1 mg
Cat No.
553030
561541 Rev. 2
Antibody Details
MR12-3
The MR12-3 monoclonal antibody specifically binds to the Vβ 13 T-cell Receptor (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V13 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype.
561541 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
561541 Rev.2
Citations & References
Development References (3)
-
Behlke MA, Chou HS, Huppi K, Loh DY. Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci U S A. 1986; 83(3):767-771. (Biology). View Reference
-
Chen D, Lee F, Cebra JJ, Rubin DH. Predominant T-cell receptor Vbeta usage of intraepithelial lymphocytes during the immune response to enteric reovirus infection.. J Virol. 1997; 71(5):3431-6. (Biology). View Reference
-
Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
561541 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
