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BD Pharmingen™ Purified Mouse Anti-human HLA-G Denatured
Clone 4H84 (RUO)
Profile of human HLA-G (denatured) reactivity on fixed, permeabilized JEG-3 cell line analyzed by flow cytometry. Fixed and permeabilized with Cat. No. 554714. Second step staining with Cat. No. 555988.
Profile of human HLA-G (denatured) reactivity on fixed, permeabilized JEG-3 cell line analyzed by flow cytometry. Fixed and permeabilized with Cat. No. 554714. Second step staining with Cat. No. 555988.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Western blot (Tested During Development)
0.5 mg/ml
AB_396753
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures
Recommended Assay Procedure:
This product is routinely tested on fixed and permeabilized (BD Cytofix/Cytoperm™, Cat. No. 554714) JEG-3 cell line by flow cytometry. It's also suitable for Western blotting and for staining acetone-fixed frozen tissue sections at 1-10 µg/ml and formalin fixed paraffin tissue sections at 1-10 µg/ml with citrate buffer pretreatment.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Purified Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555746
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
557577 Rev. 2
Antibody Details
4H84
HLA-G is a HLA class I molecule, but it is distinct from other class I molecules because of its quasi-monomorphism and restricted tissue distribution. It can be expressed as seven distinct protein forms. HLA-G1, -G2, G3, and -G4 are membrane bound while HLA-G5, -G6, and G7 are soluble. HLA-G is expressed in healthy individuals on amniocytes and cytotrophoblasts in the amnion-chorion. HLA-G interacts with inhibitory receptors such as ILT-2, ILT-4, p49 and KIR2DL4 expressed on NK cells, shifts the cytokine balance towards Th2 dominance, suppresses the proliferation of allogeneic CD4 lymphocytes and, in its soluble form, induces apoptosis of activated CD8 cells and inhibits cytolysis by NK cells.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
557577 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
557577 Rev.2
Citations & References
View product citations for antibody "557577" on CiteAb
Development References (6)
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Dorling A, Monk NJ, Lechler RI. HLA-G inhibits the transendothelial migration of human NK cells. Eur J Immunol. 2000; 30(2):586-593. (Biology). View Reference
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King A, Allan DS, Bowen M, et al. HLA-E is expressed on trophoblast and interacts with CD94/NKG2 receptors on decidual NK cells. Eur J Immunol. 2000; 30(6):1623-1631. (Biology). View Reference
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Le Bouteiller P, Blaschitz A. The functionality of HLA-G is emerging. Immunol Rev. 1999; 167:233-244. (Biology). View Reference
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Le Bouteiller P, Solier C, Proll J, Aguerre-Girr M, Fournel S, Lenfant F. Placental HLA-G protein expression in vivo: where and what for. Hum Reprod Update. 1999; 5(3):223-233. (Biology). View Reference
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Le Gal FA, Riteau B, Sedlik C, et al. HLA-G-mediated inhibition of antigen-specific cytotoxic T lymphocytes. Int Immunol. 1999; 11(8):1351-1356. (Biology). View Reference
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McMaster M, Zhou Y, Shorter S, et al. HLA-G isoforms produced by placental cytotrophoblasts and found in amniotic fluid are due to unusual glycosylation. J Immunol. 1998; 160(12):5922-5928. (Clone-specific: Immunohistochemistry, Western blot). View Reference
557577 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.