Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version
See More
BD Pharmingen™ Biotin Hamster Anti-Mouse TCR β Chain
Clone H57-597 (RUO)
Flow cytometric analysis of αβ TCR expression in spleen and thymus. BALB/c splenocytes (left panels) were simultaneously stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033), and either Biotin Hamster IgG2, λ1 Isotype Control (Cat. no. 553963; top left panel) or Biotin Hamster Anti-Mouse TCR β Chain (Cat. No. 553168; bottom left panel) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). BALB/c thymocytes (right panels) were stained with either Biotin Hamster IgG2, λ1 Isotype Control (top right panel) or Biotin Hamster Anti-Mouse TCR β Chain (bottom right panel), followed by Avidin FITC. The fluorescence histograms and contour plots were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of αβ TCR expression in spleen and thymus. BALB/c splenocytes (left panels) were simultaneously stained with PE Rat Anti-Mouse CD4 (Cat. No. 553048/553049), PE Rat Anti-Mouse CD8a (Cat. No. 553032/553033), and either Biotin Hamster IgG2, λ1 Isotype Control (Cat. no. 553963; top left panel) or Biotin Hamster Anti-Mouse TCR β Chain (Cat. No. 553168; bottom left panel) monoclonal antibodies, followed by Avidin FITC (Cat. No. 554057). BALB/c thymocytes (right panels) were stained with either Biotin Hamster IgG2, λ1 Isotype Control (top right panel) or Biotin Hamster Anti-Mouse TCR β Chain (bottom right panel), followed by Avidin FITC. The fluorescence histograms and contour plots were derived from gated events with the forward and side light-scattering characteristics of viable splenocytes and thymocytes. Flow cytometry was performed on a BD FACScan™.
Product Details
BD Pharmingen™
Tcrb; TCRbeta; TCRβ, T cell receptor beta chain
Mouse (QC Testing)
Armenian Hamster IgG2, λ1
TCR affinity-purified from mouse T-cell hybridoma DO-11.10
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported)
0.5 mg/ml
AB_394680
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures
It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2 to 4 hours prior to staining enhances the ability of anti-CD3e and anti-TCR β chain mAbs detect the T cell receptor on immature thymocytes.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Biotin Hamster Anti-Mouse TCR β Chain RUO
Size
0.5 mg
Cat No.
553169
PE Rat Anti-Mouse CD4 RUO
Size
0.1 mg
Cat No.
553048
PE Rat Anti-Mouse CD8a RUO
Size
0.1 mg
Cat No.
553032
553168 Rev. 20
Antibody Details
H57-597
The H57-597 antibody reacts with a common epitope of the β chain of the T-cell Receptor (TCR) complex on αβ TCR-expressing thymocytes, peripheral T lymphocytes, NK1.1+ thymocytes, and NK-T cells of all mouse strains tested. It does not react with γδ TCR-bearing T cells. In the fetal and adult thymus, the TCR β-chain may form homodimers or pair with the pre-TCR α-chain on the surface of immature thymocytes before TCR α-chain expression. Plate-bound or soluble H57-597 antibody activates αβ TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.
553168 Rev. 20
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553168 Rev.20
Citations & References
View product citations for antibody "553168" on CiteAb
Development References (3)
-
Gascoigne NR. Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990; 265(16):9296-9301. (Clone-specific: Immunofluorescence, Immunoprecipitation). View Reference
-
Kruisbeek AM, Shevach EM. Proliferative assays for T cell function. Curr Protoc Immunol. 2004; 3:3.12.1-3.12.14. (Clone-specific: Activation, Functional assay, Stimulation). View Reference
-
Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M. Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989; 142(8):2736-2742. (Immunogen: Activation, Flow cytometry, Functional assay, Stimulation). View Reference
553168 Rev. 20
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.
Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.