Flow Cytometry
Resources
Application Notes
General
- Standardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva™ Version 6 Software
- Selecting Reagents for Multicolor Flow Cytometry
- Construction of Multicolor Antibody Panels for the Flow Cytometric Analysis of Murine Thymic Stromal Cells
- An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
- MultiFlow Cytometry Technical Bulletin
- Analysis of Apoptotic Cells using the BD FACSArray™ Bioanalyzer
- Multicolor Flow Cytometric Analysis of S-phase (BrdU Incorporating)
Cells Using the BD FACSArray™ Bioanalyzer - Cell-Cycle Analysis Using the BD FACSArray™ Bioanalyzer
- Flow Cytometry and High-Content Imaging to Identify Markers of Monocyte-Macrophage Differentiation
- Technique for Loading Cells with BD Horizon™ Violet Proliferation Dye 450 (VPD450)
Acquisition and Analysis
Cell Subsetting
- Peripheral Blood Dendritic Cells Revealed by Flow Cytometry
- Four-Color Analysis of Bone Marrow Transplantation Specimens
Cytokine (Intracellular Staining) Sample Preparation
- Cytokine Detection in Antigen-Activated CD8+ and CD4+ T Cells
- BD FastImmune™ Assay System
- Simultaneous Detection of Proliferation and Cytokine Expression in Peripheral Blood Mononuclear Cells
- Detecting Intracellular Cytokines in Activated Lymphocytes
- Detecting Intracellular Cytokines in Activated Monocytes
Microbial
- Bacterial Detection and Live/Dead Discrimination by Flow Cytometry
- Bacterial Disinfectant Efficacy Using Flow Cytometry
- Evaluation of Yeast Viability and Concentration During Wine Fermentation Using Flow Cytometry
Sorting
- Sorting Transfected Cells Based on Gene Expression, Followed by Culture In Vitro
- Detection of X and Y Chromosomes by Fluorescence In Situ Hybridization (FISH) in Whole Cells
- Enrichment of Monocytes for Morphological Study
- In Situ Hybridization and Image Analysis on Nuclei Sorted on the Basis of DNA Content
- Isolation of Natural Killer (NK) Cells for Functional Studies
- Measurement of Cell Recovery After Sorting with a Catcher-Tube-Based Cell Sorter
- Reduction in Endotoxin Levels After Performing the Prepare for Aseptic Sort Procedure on the BD FACSAria™ II Flow Cytometer
- Detection of Cryptosporidium Oocysts and Giardia Cysts in Water
- BD FACService™ TechNotes
Brochures
Catalogs
Product Information Sheets
Product List
Protocols
General
Acquisition and Analysis
Apoptosis
- Annexin V Staining Protocol
- APO-BRDU™ Procedure
- APO-DIRECT™ Procedure
- Induction of Apoptosis by Treatment with Camptothecin
- Induction of Apoptosis by Treatment with Staurosporine
- Receptor-Mediated Apoptosis in Mouse Cells Using Anti-Fas/CD95 (Clone Jo2)
- Receptor-Mediated Apoptosis in Human Cells Using Anti-Fas/CD95 (Clone DX2)
Cell Cycle/DNA Analysis
- Staining Procedure for Flow Cytometric Detection of Human Cyclins
- Flow Cytometry Staining Protocol for Detection of Ki-67
- Detection of BrdU Incorporation in DNA Synthesizing Cells
Cytokine (Intracellular Staining) Sample Preparation
Human Sample Preparation
- Direct Immunofluorescence Staining of Mononuclear Cells
- Direct Immunofluorescence Staining of Whole Blood Using a Lyse/No-Wash Procedure
- Direct Immunofluorescence Staining of Whole Blood Using a Lyse/Wash Procedure
- Isotype Control Antibody Selection Guide
- Cell Surface Staining of Human PBMCs and Suspension Cell Lines
- Platelet Activation, Staining, and Analysis
- Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
- Indirect Immunofluorescence Staining of Human Platelets
- Indirect Immunofluorescence Staining of Mononuclear Cells
- Intracellular Staining of Human Red Blood Cells
- Surface Staining of Human Red Blood Cells
Mouse/Rat Sample Preparation
Tools
Webinars
- Multicolor Flow Cytometry: Principles of Panel Design
- Instrument Characterization and Performance Tracking for Digital Flow Cytometers
Cy™ is a trademark of Amersham Biosciences Corp.
Multicolor Flow Cytometry