PE Rat Anti-Mouse DCIR1 (Clec4a2)
Clone TKKT-1 (RUO)
- Brand BD Pharmingen™
- Alternative Name Clec4a; Clec4a2; Clecsf6; DCIR; Dcir1
- Concentration 0.2 mg/ml
- Isotype Rat LEW, also known as Lewis IgG2a, κ
- Reactivity Mouse (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen Mouse DCIR1 Transfected Cell Line
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The TKKT-1 monoclonal antibody specifically recognizes Dendritic cell immunoreceptor 1 (DCIR1), a single-pass type II membrane glycoprotein that is encoded by Clec4a2 (C-type lectin domain family 4, member a2). DCIR1 (Clec4a2) is a member of the dendritic cell immunoreceptor (DCIR) family which contains four dendritic cell inhibitory receptors (DCIR1-4) and two activating receptors (DCAR1, 2). The C-terminal extracellular region of DCIR1 (Clec4a2) has one C-type lectin carbohydrate recognition domain (CRD) that is followed by a transmembrane region. Its cytoplasmic tail contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that can transmit inhibitory signals. DCIR1 (Clec4a2) is expressed on myeloid cells including dendritic cells, macrophages, neutrophils and eosinophils. C-type lectins bind to carbohydrates in a calcium-dependent manner. This inhibitory receptor may be involved in the regulation of immune responses.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.