- Brand BD Horizon™
- Concentration 0.1 mg/ml
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
BD Horizon BV480 Streptavidin is a useful detection reagent for labeling biotinylated primary or secondary antibodies for detection of antigens by immunofluorescence. Streptavidin has very high binding affinity for biotin, which is conjugated to the antibody.
Streptavidin was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye has been exclusively developed by BD Biosciences as an optimal dye for imaging applications. BD Horizon BV480 has an Ex Max at 436 nm and Em Max at 477 nm. The use of a mounting reagent (eg, ProLong® Gold) is highly recommended to maximize the photostability of BV480.
For confocal microscopy systems, a 440 nm laser is the optimal excitation source with optimal emission collection centered at 477 nm. BV480 fluorescence can also be excited well by 405 nm and 458 nm lasers.
For epifluorescence microscopes with broad spectrum excitation sources, the recommended excitation and emission filters are 445/20 nm and 485/20 nm bandpass filters, respectively. For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user. For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging.
BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40-nm) filter set. BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510. Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Streptavidin was conjugated with BD Horizon BV480 under optimum conditions, and unconjugated streptavidin and free BD Horizon BV480 were removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
- Triton is a trademark of the Dow Chemical Company.
- ProLong® is a registered trademark of Thermo Fisher Scientific, Inc. Waltham, MA.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.