Biotin Rat Anti-Mouse IgG1
Clone A85-1 (RUO)
- Brand BD Pharmingen™
- Alternative Name Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4
- Concentration 500 µg/ml
- Isotype Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
- Reactivity Mouse (QC Testing)
ELISA (Routinely Tested)
Immunohistochemistry-formalin (antigen retrieval required), Flow cytometry, Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
- Immunogen Pooled Mouse IgG1
- Storage Buffer Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.
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Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
For immunohistochemical staining of acetone-fixed frozen sections, a dilution range of 1:50 - 1:200 is suggested.