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    BV421 Mouse Anti-Cardiac Troponin T
    BV421 Mouse Anti-Cardiac Troponin T
    Flow cytometric analysis of Cardiac Troponin T in mouse cardiomyocytes differentiated in vitro. The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438, dashed line) or BD Horizon BV421 Mouse Anti-Cardiac Troponin T (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD LSR™ II flow cytometer system.
    BV421 Mouse Anti-Cardiac Troponin T
    Immunohistofluorescent analysis of cardiac troponin T expression by cells within C57BL/6 mouse heart. A mouse heart cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1% Triton™ X-100, blocked with 5% goat serum and 1% BSA diluted in 1× PBS, and stained with BD Horizon™ BV421 Mouse Anti-Cardiac Troponin T (Cat. No. 565618, pseudo-colored green). The section was then counterstained with BD Pharmingen™ DRAQ5™ (Cat. No. 564903, pseudo-colored red). The images were captured on a standard four-laser confocal microscope. Original magnification, 40×.
    BV421 Mouse Anti-Cardiac Troponin T BV421 Mouse Anti-Cardiac Troponin T
    Flow cytometric analysis of Cardiac Troponin T in mouse cardiomyocytes differentiated in vitro. The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438, dashed line) or BD Horizon BV421 Mouse Anti-Cardiac Troponin T (solid line). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD LSR™ II flow cytometer system.
    Immunohistofluorescent analysis of cardiac troponin T expression by cells within C57BL/6 mouse heart. A mouse heart cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with 0.1% Triton™ X-100, blocked with 5% goat serum and 1% BSA diluted in 1× PBS, and stained with BD Horizon™ BV421 Mouse Anti-Cardiac Troponin T (Cat. No. 565618, pseudo-colored green). The section was then counterstained with BD Pharmingen™ DRAQ5™ (Cat. No. 564903, pseudo-colored red). The images were captured on a standard four-laser confocal microscope. Original magnification, 40×.
    Product Details
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    BD Horizon™
    cTnT, Cardiac Muscle Troponin T, Troponin T type 2, TNNT2
    Mouse (QC Testing), Human (Tested in Development), Rat, Pig, Dog, Chicken, Rabbit, Guinea Pig (Reported)
    Mouse BALB/c IgG1, κ
    Rabbit cardiac troponin T Protein
    Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence (Tested During Development)
    0.2 mg/ml
    AB_2739306
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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    Recommended Assay Procedures

    For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Triton is a trademark of the Dow Chemical Company.
    4. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    5. DRAQ5™ is a registered trademark of BioStatus Ltd.
    6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    9. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
    10. An isotype control should be used at the same concentration as the antibody of interest.
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    565618 Rev. 2
    Antibody Details
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    13-11

    The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels.  Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells.

    565618 Rev. 2
    Format Details
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    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    565618 Rev.2
    Citations & References
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    Development References (7)

    1. Jáchymová M, Muravská A, Paleček T, et al. Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy. Physiol Rev. 2012; 61(2):169-175. (Biology). View Reference
    2. Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012; 109(27):E1848-E1857. (Clone-specific: Flow cytometry). View Reference
    3. Malouf NN, McMahon D, Oakeley AE, Anderson PA. A cardiac troponin T epitope conserved across phyla. J Biol Chem. 1992; 267(13):9269-9274. (Immunogen: Electron microscopy, ELISA, Immunofluorescence, Western blot). View Reference
    4. Mauritz C, Schwanke K, Reppel M, et al. Generation of functional murine cardiac myocytes from induced pluripotent stem cells. Circulation. 2008; 118(5):507-517. (Clone-specific: Immunofluorescence). View Reference
    5. Thierfelder L, Watkins H, MacRae C, et al. Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere.. Cell. 1994; 77(5):701-12. (Biology). View Reference
    6. Uosaki H, Fukushima H, Takeuchi A, et al. Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression. PLoS ONE. 6(8)(Clone-specific: Flow cytometry). View Reference
    7. Zhang J, Wilson GF, Soerens AG, et al. Functional cardiomyocytes derived from human induced pluripotent stem cells. Circ Res. 104(4)(Clone-specific: Immunofluorescence). View Reference
    View All (7) View Less
    565618 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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