PE Hamster IgG2, κ Isotype Control
Clone B81-3 (RUO)
- Brand BD Pharmingen™
- Alternative Name Anti-KLH
- Concentration 0.2 mg/ml
- Isotype Armenian Hamster IgG2, κ
Flow cytometry, Isotype control (Routinely Tested)
Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
- Immunogen Keyhole Limpet Hemocyanin
- Storage Buffer Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The B81-3 monoclonal antibody was raised against keyhole limpet hemocyanin (KLH), an antigen not expressed by mammalian cells or cell lines. Intended for use as a hamster immunoglobulin isotype control, the B81-3 antibody can be used as a negative control to help differentiate non-specific background signal when compared against a signal from a specific hamster antibody. The purified B81-3 antibody may also serve as a useful standard for some ELISA applications. KLH is a oxygen carrying metalloprotein from a species of keyhole limpet that lives off the coast of California and is often used as a carrier protein to help elicit immune responses to haptens. Since KLH is phylogenetically distant from many mammalian proteins, it often has very minimal to no cross-reactivity for many immunological assays.
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
This product is not recommended for use as an isotype control for intracellular or immunohistochemical staining applications.