Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Middle East / Africa (English)
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
    Alert Icon
    BV421 Rat Anti-Mouse CD121b
    BV421 Rat Anti-Mouse CD121b
    Flow cytometric analysis of CD121b expression on mouse bone marrow cells. BALB/c bone marrow cells were stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD121b antibody (Cat. No. 562926, Right Panel). The two-parameter flow cytometric dot plots show the correlated expression patterns of side-scattered light (SSC) characteristics versus CD121b (or Ig Isotype control staining) for gated events with the light scattering characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
    BV421 Rat Anti-Mouse CD121b
    Flow cytometric analysis of CD121b expression on mouse bone marrow cells. BALB/c bone marrow cells were stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602, Left Panel) or BD Horizon™ BV421 Rat Anti-Mouse CD121b antibody (Cat. No. 562926, Right Panel). The two-parameter flow cytometric dot plots show the correlated expression patterns of side-scattered light (SSC) characteristics versus CD121b (or Ig Isotype control staining) for gated events with the light scattering characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
    Product Details
    Down Arrow Up Arrow


    BD Horizon™
    Il1r2; IL-1RII; IL-1R2; Il1rb; IL-1Rβ; IL-1R-beta; IL-1 receptor beta chain
    Mouse (QC Testing)
    Rat LEW, also known as Lewis IgG2a, κ
    Mouse IL-1RII Protein
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_2737896
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
    Show More blue down arrow Show Less blue up arrow

    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    3. An isotype control should be used at the same concentration as the antibody of interest.
    4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    7. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
    8. Brilliant Violet™ 421 is a trademark of Sirigen.
    Show More blue down arrow Show Less blue up arrow
    562926 Rev. 1
    Antibody Details
    Down Arrow Up Arrow
    4E2

    The 4E2 monoclonal antibody specifically binds to CD121b which is also known as the IL-1 receptor type II (IL-1RII, IL-1R2) or IL-1 receptor beta (IL-1R-beta, IL-1Rβ). CD121b is encoded by the Il1r2 gene and presents as a 68 kDa type I transmembrane glycoprotein. It is expressed on a variety of cell types including B cells, neutrophils, monocytes, and bone marrow progenitor cells. A soluble form of CD121b is produced by proteolysis especially after cellular activation. Unlike the IL-1 type I receptor (CD121a), CD121b has a short cytoplasmic domain and does not mediate IL-1 signaling. CD121b may function in a membrane or soluble form as a decoy receptor that can bind IL-1 and reduce its effective concentration and biological activity. The 4E2 antibody blocks IL-1 binding to the IL-1 receptor type II.

    The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes.  With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421  can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421  conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

    562926 Rev. 1
    Format Details
    Down Arrow Up Arrow
    BV421
    The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    BV421
    Violet 405 nm
    407 nm
    423 nm
    562926 Rev.1
    Citations & References
    Down Arrow Up Arrow

    Development References (7)

    1. Colotta F, Re F, Muzio M, et al. Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4. Science. 1993; 261(5120):472-475. (Biology). View Reference
    2. Cumberbatch M, Dearman RJ, Kimber I. Characteristics and regulation of the expression on interleukin 1 receptors by murine Langerhans cells and keratinocytes. Arch Dermatol Res. 1998; 290(12):688-695. (Clone-specific: Flow cytometry). View Reference
    3. Hestdal K, Ruscetti FW, Chizzonite R, et al. Interleukin-1 (IL-1) directly and indirectly promotes hematopoietic cell growth through type I IL-1 receptor. Blood. 1994; 84(1):125-132. (Clone-specific). View Reference
    4. Hunter CA, Chizzonite R, Remington JS. IL-1 beta is required for IL-12 to induce production of IFN-gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. J Immunol. 1995; 155(9):4347-4354. (Clone-specific: Neutralization). View Reference
    5. McMahan CJ, Slack JL, Mosley B, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J. 1991; 10(10):2821-2832. (Biology). View Reference
    6. Oldenburg HS, Pruitt JH, Lazarus DD, et al. Interleukin 1 binding to its type I, but not type II receptor, modulates the in vivo acute phase response. Cytokine. 1995; 7(6):510-516. (Clone-specific). View Reference
    7. Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
    View All (7) View Less
    562926 Rev. 1

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

    Website Feedback