PE Rat Anti-Human CX3CR1
Clone 2A9-1 (RUO)
- Brand BD Pharmingen™
- Alternative Name CCRL1; CMKBRL1; CMKDR1; GPR13; GPRV28; V28; Fractalkine Receptor
- Vol. Per Test 5 µl
- Isotype Rat IgG2b, κ
- Reactivity Human (QC Testing)
- Application
Flow cytometry (Routinely Tested)
- Immunogen Human CX3CR1 Recombinant Protein
- Entrez Gene ID 1524
- Storage Buffer Aqueous buffered solution containing BSA and ≤ 0.2% sodium azide
- Regulatory Status RUO
Regulatory Status Legend
Description
The 2A9-1 monoclonal antibody specifically binds to human CX3CR1, which is also known as chemokine (C-C) receptor-like 1 (CCRL1), Beta chemokine receptor-like 1 (CMK-BRL-1), G protein-coupled receptor 13 (GPR13), or GPRV28 (V28). CX3CR1 is a seven transmembrane G protein coupled receptor that is expressed by NK cells, T cells, and monocytes. The cellular expression of CX3CR1 is correlated with high levels of intracellular perforin and granzyme B. CX3CR1 serves as a receptor for fractalkine (CX3CL1). Fractalkine is a transmembrane chemokine of the CX3C family that is expressed on activated endothelial cells, neurons, and astrocytes. Interaction of CX3CR1 with fractalkine initiates cellular adhesive and chemotactic responses.
Format
- Format PE
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Suggested Companion Products
PE Rat IgG2b, κ Isotype Control RUO
0.1 mg
Cat No: 553989
Alexa Fluor® 488 Mouse Anti-Human CD3 UCHT1 (also known as UCHT-1; UCHT 1) RUO
100 Tests
Cat No: 557694
BUV737 Mouse Anti-Human CD56 NCAM16.2 (also known as NCAM 16) RUO
100 Tests
Cat No: 564447
PE Rat Anti-Human CX3CR1 2A9-1 RUO
25 Tests
Cat No: 565798
Lysing Buffer RUO
100 mL
Cat No: 555899
Lysing Solution 10X Concentrate CE/IVD
100
Cat No: 349202
Stain Buffer (FBS) RUO
500 mL
Cat No: 554656
Stain Buffer (BSA) RUO
500 mL
Cat No: 554657
Resources & Tools | ||||||
---|---|---|---|---|---|---|
Spectrum Viewer | Panel Designer | Spectrum Viewer | Regulatory Document Website |
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.